Prezygotic reproductive isolation between Saccharomyces cerevisiae and Saccharomyces paradoxus

Table 2 Mate choice information table

Pair	"chooser" strain	Total trials as "chooser"	Total zygotes formed	Observed number of hybrid zygotes	Expected number of hybrid zygotes^a	Hybrid zygote χ² (p value)^b	Always own species χ² (p value)^c	Mating propensity^d	Mating Prospensity χ² (p value)
1	Sc1	300	115	49	76.67	28.89 (<0.001)	4.882943 (0.0271)	51.11	1.035 (0.3089)
	Sp1	206	69	34	46	8.63 (0.0033)	8.628882 (0.0033)	44.66
2	Sc2	270	88	28	58.67	31.82 (<0.001)	0.035264 (0.8510)	43.46	2.212 (0.1369)
	Sp2	220	85	47	56.67	4.45 (0.0349)	19.4555 (<0.0001)	51.51
3	Sc3	250	70	22	46.67	31.42 (<0.001)	0.044643 (0.8327)	37.33	0.164 (0.6857)
	Sp3	344	90	42	60	15.31 (<0.001)	7.8125 (0.0052)	34.88
4	Sc4	210	78	25	52	40.51 (<0.001)	0.014423 (0.9044)	49.52	0.0046 (0.9462)
	Sp4	220	80	38	53.33	12.38 (<0.001)	7.876563 (0.005)	48.48
5	Sc5	240	90	26	60	56.13 (<0.001)	0.877974 (0.3488)	50.00	2.229 (0.1355)
	Sp5	260	80	33	53.33	22.13 (<0.001)	2.626563 (0.1051)	41.03

Table showing the raw data obtained from the mate choice trials as well as the hybrid zygote and mating propensity χ² values.
^a– The expected number of hybrid zygotes if no mating preference exists between the two species (2/3 total – see Methods)
^b– χ² test on the number of hybrid zygotes observed compared to the number expected if there is no mate preference (Yates corrected) (see also Figure 2).
^c– χ² test on the number of hybrid zygotes observed compared to the 1/3 expected under the "always choose own species" model. (see Figures 1 and Methods)
^d– Percentage of mating trials producing zygotes after correcting for the 25% of trials that cannot form zygotes because all three spores are the same mating type (see Methods).).
^e– χ² test for the effect of species in pair on mating propensity (Yates corrected).

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