Figure 6

Example of the effect of biological filters in coevolution analyses. In this case study we have used the heat-shock protein GroEL, which functional domains are well characterised. The different domains (apical, intermediate and equatorial) are identified in the cartoon representing the linear GroEL sequence in red, green and yellow colours, respectively. G1 to G6 blocks show the sites under coevolution belonging to each coevolution group as coloured bars. Sites belonging to the same group of coevolution are shown in the crystal structure of one of the GroEL subunits (PDB accession number: 1svt.pdb) and are coloured following the same pattern as in the blocks of the groups of coevolution.