Figure 4

AmSoxB1 mRNA is localised in honeybee ooctyes and is expressed in the ventral neuroectoderm during honeybee embryogenesis. A. Ovariole from honeybee queen was stained for AmSoxB1 mRNA. B. A higher magnification image of AmSoxB1 expression in the queen oocyte. AmSoxB1 mRNA is detected in the posterior nurse (N) cells and in the neighbouring oocyte, where the RNA becomes localized to the dorsal side of the oocyte (O; arrow). C. Ventral view of stage 6 embryo showing staining for AmSoxB1 along the gastrulation folds in the ventral neuroectoderm (VNE). D. Side view of later stage 6 embryo, AmSoxB1 is strongly expressed in the procephalic neuroectoderm. E. AmSoxB1 is expressed in the neuroblasts along the ventral midline at stage. AmSoxB1 expression in the cephalic brain lobes. F. In later stage embryos, neuronal expression of AmSoxB1 continues along ventral midline in neurons that radiate from the ventral nerve cord. Side view of the same embryo (G). Scale bar is 100 μm.