Research article | Open | Published:
The repertoire of G protein-coupled receptors in the sea squirt Ciona intestinalis
BMC Evolutionary Biologyvolume 8, Article number: 129 (2008)
G protein-coupled receptors (GPCRs) constitute a large family of integral transmembrane receptor proteins that play a central role in signal transduction in eukaryotes. The genome of the protochordate Ciona intestinalis has a compact size with an ancestral complement of many diversified gene families of vertebrates and is a good model system for studying protochordate to vertebrate diversification. An analysis of the Ciona repertoire of GPCRs from a comparative genomic perspective provides insight into the evolutionary origins of the GPCR signalling system in vertebrates.
We have identified 169 gene products in the Ciona genome that code for putative GPCRs. Phylogenetic analyses reveal that Ciona GPCRs have homologous representatives from the five major GRAFS (Glutamate, Rhodopsin, Adhesion, Frizzled and Secretin) families concomitant with other vertebrate GPCR repertoires. Nearly 39% of Ciona GPCRs have unambiguous orthologs of vertebrate GPCR families, as defined for the human, mouse, puffer fish and chicken genomes. The Rhodopsin family accounts for ~68% of the Ciona GPCR repertoire wherein the LGR-like subfamily exhibits a lineage specific gene expansion of a group of receptors that possess a novel domain organisation hitherto unobserved in metazoan genomes.
Comparison of GPCRs in Ciona to that in human reveals a high level of orthology of a protochordate repertoire with that of vertebrate GPCRs. Our studies suggest that the ascidians contain the basic ancestral complement of vertebrate GPCR genes. This is evident at the subfamily level comparisons since Ciona GPCR sequences are significantly analogous to vertebrate GPCR subfamilies even while exhibiting Ciona specific genes. Our analysis provides a framework to perform future experimental and comparative studies to understand the roles of the ancestral chordate versions of GPCRs that predated the divergence of the urochordates and the vertebrates.
On a taxonomic and phylogenetic scale, Ciona intestinalis is a protochordate belonging to the ascidian class of urochordates that diverged from a lineage leading to the vertebrates approximately 520 million years ago . This extant ascidian occupies a crucial place in the "Tree of life" as an out-group to the vertebrates and hence studies addressing evolutionary aspects of Ciona have the potential to offer insight into some of the most intriguing questions about the origin of the vertebrates from a chordate lineage. Recent genomic analysis has shown that the urochordates, and not cephalochordates, are the closest extant relatives of vertebrates . As an added advantage, this ascidan is also amenable to experimentation and even has a century old history of being used for embryological studies . A translucent morphology, availability of developmental mutants, established transgenic experimental procedures, EST databases and quickly spawning embryos are just a few advantages that make Ciona a favourite model system for developmental biologists. Ciona has a compact genome size of about 160 million base pairs and contains approximately 16,000 protein-coding genes .
GPCR based signal transduction is ubiquitous in eukaryotic genomes and forms the basis of detection of diverse environmental cues such as odorant molecules, amines, peptides, lipids, nucleotides and photons. A common structural feature of GPCRs is the presence of a highly conserved architecture of seven stretches of transmembrane spanning residues linked by alternate extracellular and intracellular loops. The diversity among GPCRs primarily stem from the presence of characteristic N-terminal extracellular domains and C-terminal intracellular domains and to a relatively lesser extent from the connecting loops which share limited sequence similarity. A number of fully sequenced genomes have been mined for their repertoire of GPCRs and comparative phylogenetic studies described for Homo sapiens , Tetraodon nigrovirdis , Anopheles gambiae , Gallus gallus , Rattus rattus , Mus musculus , Drosophila melanogaster  and Branchiostoma floridae .
On the basis of a large scale phylogenetic analysis, GPCRs in human and subsequently in many other genomes have been classified into five major families (GRAFS);Glutamate (G), Rhodopsin (R), Adhesion (A), Frizzled/Smoothened (F), and Secretin (S) [5, 6, 8–12]. We denote the family names in italic with an initial capital letter to avoid possible confusion with for example, the rhodopsin receptor. HMMs (Hidden Markov Models) derived from the GRAFS classification system, along with the HMMs of other non-GRAFS families of GPCRs have been used to tract putative GPCRs from 13 completely sequenced genomes (including Ciona) into specific families and subfamilies. The analysis revealed that GRAFS families can be found in all bilateral species suggesting that they arose before the split of nematodes from the chordate lineage . A recent review details progress in mining the gene repertoire and expressed sequence tags (ESTs) for GPCRs in several completed genomes .
The Ciona genome provides scope for identification of GPCRs that are analogous in function to its vertebrate counterparts. This provides a resource for comparative analysis aimed at assigning function to putative gene products . Furthermore, comparative protein domain analysis as implemented in evolutionary trace methods can identify functional interface residues essential for ligand binding and subsequent signalling events . Identification of conserved domains and/or novel GPCRs and their ligands are of major interest in light of the well-recognized roles of GPCRs in clinical medicine [17–19]. Furthermore, the repertoire of GPCRs in Ciona could provide insights into some of the intriguing questions in evolutionary biology about the origins and evolution of the GPCR signalling system in a protochordate and its further diversification into the vertebrate lineage. Ciona possesses organ systems are homologous to vertebrate heart, thyroid, notochord and pineal systems [20, 21]. Our comparative genomic analysis could now serve as a basis for carrying out molecular genetic studies in Ciona to address many functional and regulatory aspects of GPCR associated vertebrate organ development and physiology. Finally, it is also important to note that such genome-wide comparisons may provide some wrong conclusions about orthologous relationships in individual cases and results from such analysis are best used as complementary tools for carrying out specific experimental studies.
Here, we describe the repertoire of GPCRs in the Ciona intestinalis genome and provide a comparative perspective to the GRAFS families, including a detailed analysis of the LDLRR-GPCR (Low-density lipo-protein receptor repeat containing GPCR) family in Ciona.
Our aim was to generate an independent data set of the repertoire of Ciona GPCRs and thereupon, using phylogenetic approaches provide a comparative genomic perspective of the salient features observed in the repertoire. In order to generate the complete set of putative GPCRs, we adopted a comprehensive strategy wherein predicted 6/7/8 transmembrane receptor sequences identified from the JGI (Joint Genome Institute, USA) Ciona proteome was subjected to an array of similarity and pattern search analysis for GPCR specific features. The hits from these search methods were further subjected to phylogenetic analyses (Figure 1). Such a process identified 169 putative GPCRs that represent ~1.1 % of the total number of gene products predicted from the Ciona genome. The proportion of GPCRs to the genome size is therefore comparable to those predicted in vertebrate and insect genomes . To the best of our knowledge, manual searches revealed only 30 unique Ciona GPCR sequences to be present in the current updated public sequence repositories. The Ciona GPCRs identified are summarized in Table 1 and Table 2. The complete list of Ciona GPCR sequences identified through this study is available as Additional data file 1.
HMM based genome-wide GPCR surveys have been previously carried out in 13 genomes, including that of Ciona . While this HMM based study provided a broad overview of the repertoire of GPCRs in the Ciona genome, an independent large-scale molecular phylogenetic treatment with respect to the tunicate GPCRs has not emerged and awaits investigation. GPCR features reflecting highly specialized ascidian specific biology are usually evident from such phylogenetic analysis. Of particular interest are whole-genome orthology and paralogy comparisons that are not evident from the previous HMM based search approach . A table describing orthology and paralogy observations identified in Ciona GPCRs through our comparative genomic studies is provided [Additional data file 2: sheet 1]. Our phylogenetic study also provides the first ever insight into Ciona specific protochordate GPCR repertoire apart from vindicating the presence of the five major GRAFS families (Figure 2). Homologs of the chemosensory GPCRs from nematodes, plant GPCRs, Yeast Pheromone receptors, gustatory and olfactory receptors from insects were not identified. 66 clear orthologs of human GPCRs could be identified in Ciona based on phylogenetic analysis.
Since EST evidence covers three quarters of the predicted Ciona genes, we also identified EST matches for the 169 putative GPCRs using TBLASTN with a stringent cut off E-value of 1e-15. Except for 12 of the 169 sequences, the rest of the GPCRs had at least one EST match. EST matches for these receptors were sorted as being derived from different developmental stages including fertilized egg, tailbud embryo, larval, embryo, gastrula and neurula, cleavage/cleaving stage, Stage 3, directional larva, young adult, juvenile and mature adult stage as well as adult tissues like heart, neural complex, gonad, digestive, blood/haemocytes, endostyle and testis [Additional data file 2: sheet 2]. A preliminary observation from the EST table also showed that as many as 133 receptors had at least one match that could be recovered in the ten different developmental stages of Ciona at the cut off E-value of 10-15, suggestive of a role for these GPCRs in developmental processes.
The RhodopsinFamily (116 members)
The Rhodopsin family in Ciona includes 116 receptors that constitute about 68% of the entire GPCR repertoire. The Rhodopsin family has analogous subfamily level representatives among the four main groups, termed α-, β-, γ-, and δ-group, which in turn are subdivided into 13 major subfamilies in humans . At the individual gene level, 39 out of the 116 Rhodopsin receptors display a clear human ortholog. However, unlike in the human Rhodopsin family, distinct clustering into the four major groups was not observed in Ciona. This is only expected since it is known that such grouping or relatedness is very restricted as regards species content.
Our large-scale phylogenetic analysis of Ciona Rhodopsin family with annotated receptors from other genomes revealed the presence of 9 of the 13 subfamilies described for the human Rhodopsin family (Figure 3). One noteworthy finding in the Ciona Rhodopsin repertoire is the identification of an innovative low-density lipoprotein receptor repeat containing GPCR cluster (LDLRR-GPCR). The LDLRR-GPCRs, the Ciona LGR-like sequences (Leucine rich repeat containing GPCRs) and the GLHRs (Glycoprotein hormone receptor) clustered into one main branch with a high bootstrap support (999 out of 1000 NJ trees) (Figure 3). For the sake of clarity in discussions, the Rhodopsin phylogenetic representation is split into two subsets, one comprising the LDLRR-GPCR/LGR-like set of receptors and the other consisting of the non-(LDLRR-GPCR/LGR-like) cluster. Among the non-(LDLRR-GPCR/LGR-like) set of sequences, the melanocortin receptors of the α-group, the classical chemokine receptors (CCRs, CXCRs), melanocyte-concentrating hormone (MCHR) subfamilies of the γ-group and the purine (PUR), MAS-related receptor (MRG) and olfactory subfamilies of the δ-group are conspicuously absent. As many as 23 receptors could not be included in any subfamily with significant phylogenetic support. These sequences were thus designated as "Other Rhodopsins". Rhodopsin subfamily members that could not be annotated at the individual gene level due to lack of support via cross-genome phylogenetic clustering were deemed as "Unclassified Rhodopsins". The "Unclassified Rhodopsins" are different from the "Other Rhodopsins" in that most of these "Unclassified Rhodopsins" could be included into individual Rhodopsin subfamilies when analyzed with limited number of sequences, but in a larger data set do not give stable or consistent topology. Since several of these "Unclassified Rhodopsins" and "Other Rhodopsins" appear to be Ciona specific and were divergent from the Rhodopsin subfamilies in other genomes, they were excluded from the final phylogenetic representation to avoid tree artefacts.
The LDLRR/LGR-like/GLHR cluster (32 members)
Ciona shows a novel lineage specific expansion and innovation in the LDLRR-GPCR/LGR-like/GLHR Rhodopsin cluster with 32 receptors, 30 of which are similar to the human INSL3/relaxin binding GPCRs in their TM regions. Of the 32 receptors in this cluster, two sequences were found to be LGR-like (ci0100148288 and ci0100151424), with Leucine-rich repeat domains (LRR) observable in their N-termini. The sequence ci0100148288 possesses five LRR domains and was identified as a candidate INLS3/Relaxin GPCR-like based on conserved domain database searches. The sequence ci0100151424 is an N-terminal LRR containing GPCR that does not bear any significant similarity to INSL3/Relaxin binding GPCRs in its TM region. One glycoprotein hormone receptor could be recovered (ci0100133821) and was identified as an ortholog of LHCGR (Lutenizing hormone/chorio-gonadotropin receptor).
It is noteworthy that as many as 14 out of the 32 receptors apart from bearing sequence similarity to INSL3/relaxin binding GPCRs in their TM region, comprise of a low density lipoprotein receptor Class A domain (LDL-A) represented either singly or in 2–10 multiple tandem repeats at the N-termini [Additional data file 3]. In contrast to other vertebrate LGRs (LGR7/LGR8) where a single LDL-A module at the N terminus of the ectodomain is followed by multiple LRRs and 7 TM helices, the 14 Ciona LDLRR-GPCR family members lack LRRs  [Additional data file 3]. Also notable is the fact that this unique domain organisation of LDL-A domain tandem repeats in Ciona has been reported only in snail LGR that contains tandem repeats of LDL-A domains in addition to multiple LRR domains  [Swiss-Prot: GR101_LYMST]. 14 additional sequences belonging to this subfamily possess shorter N-terminal regions that do not contain any explicitly recognizable domains while bearing INSL3/Relaxin binding GPCR-like TM regions [Additional data file 3]. These sequences are therefore not authentic N-terminal associated "Leucine Rich Repeat containing GPCRs" and hence been designated as "Unclassified LGR-like". It is possible that the 14 "Unclassified LGR-like" sequences are fragmentary or incompletely modelled at the N-termini. At least one instance of an incorrectly aligned domain architecture in a LDL-A domain bearing GPCR was revealed (ci0100131758) after a BLAT of the sequence was performed against the Ciona genome. A proposed correct model is provided in the Additional data file 1.
A phylogenetic analysis was carried out to resolve the relationship between the human LGRs (LGR 7/LGR 8), the snail LGR, the 14 Ciona LDLRR-GPCR family members (excluding an incorrectly aligned member: ci0100131758) and the one candidate INSL3/Relaxin binding GPCR-like sequence (ci0100148288). The unifying theme among all these receptors is their INSL3/Relaxin binding GPCR-like TM region. This phylogenetic tree was rooted using a human LHCGR and a Ciona orphan LGR-like (ci0100151424) that are distantly related to these sequences [Figure 4]. The receptors in this data set were truncated to include only the sequence of the intervening TM 1- TM 6/7 region. This truncation was carried out to eliminate the variabilities in the number of LDL-A and LRR tandem repeats.
Phylogenetic analysis shows that the human LGRs, the snail LGR, the 14 Ciona LDLRR-GPCRs and the one Ciona INSL3/Relaxin GPCR-like (ci0100148288) clustered into one group separately from the distantly related human LHCGR and the Ciona LGR-like outgroup suggestive of a common evolutionary origin to the INSL3/Relaxin GPCR-like 7 TM region. Furthermore, the domain analysis also suggests that the 14 Ciona LDLRR-GPCRs and the one candidate Ciona INSL3/Relaxin GPCR-like seem to have evolved independently of the human LGRs and the snail LGR in the non-TM N-terminal regions.
The Glutamatefamily (8 members)
Many Glutamate (GLR) receptors are crucial modulators involved in neurotransmission. Most of these receptors have long N-termini that serve to bind a cognate ligand . The Ciona Glutamate family clustered with a strong bootstrap support (998/1000) separating the cluster from its closest neighbours (Figure 2). Phylogenetic analysis revealed that the Ciona genome contains 8 members of the Glutamate family. These receptors represent all of the GLR subfamilies with the exception of Taste (TAS1) receptors. Human orthologs for Calcium-sensing receptor (CASR), Retinoic acid inducible GPCR (RAIG), Metabotropic glutamate receptor (GRM) and GABA receptors could be identified in Ciona with significant sequence similarity. Among the Ciona GLRs, the CASR (ci0100130340) homolog displayed the highest identity to the human counterpart CASR (34.7%). ESTs for the metabotropic GABAB related receptor (ci0100152670) could be retrieved from the neural-complex tissues in Ciona, suggesting similar roles for this receptor in synaptic function as proposed in vertebrates [Additional data file 2: sheet 2].
The Secretinfamily (8 members)
The Secretin family includes receptors for calcitonin (CALCR), vasoactive intestinal peptide (VIPR2), glucagon-like peptide (GLPR), pituitary adenylate cyclase-activating polypeptide (PACAP), the parathyroid hormone receptor (PTHR) and several other related peptides as well as hormones . The Ciona Secretin family received very high bootstrap support (999 out of 1000 NJ trees) (Figure 2). 8 GPCRs could be identified in Ciona and are related to the vertebrate Secretin family with which they shared high sequence similarity. The phylogenetic clustering also supports possible specific gene duplication events for three pairs of sequences, evident from the high bootstrap support (>80%) for the tree nodes for the pairs (ci0100139945/ci0100151327), (ci0100145252/ci0100145281), and (ci0100145584/ci0100145837). This is consistent with the recent report on the evolution of Secretin family receptors in Ciona . The gene pair (ci0100139945/ci0100151327) clusters with the PTHR subfamily of receptors, while the pair (ci0100145252/ci0100145281) clusters with GLPR group. The remaining pair (ci0100145584/ci0100145837) clusters with the CRHR group, whereas ci0100141310 and ci0100141557 appear to be orthologs of GLPR and CALCR/CALCRL respectively (Figure 2). Among Secretin receptors, the Ciona PTHR homologs (ci0100139945/ci0100151327) showed the highest sequence identity (37% identity to PTHR1 in both cases) to their human counterparts (Additional file 4).
The Frizzled/Smoothenedfamily (4 members)
The Ciona proteome data set contains 4 members of the Frizzled/Smoothened GPCR family each of which have at least 6 TM regions. The Ciona Frizzled receptor cluster branched out with a very high bootstrap support (100%) in NJ trees (Figure 2). Frizzled receptors have been shown to couple with G-proteins after activation by Wnt, a glycoprotein ligand and are known to play a key role in tissue-polarity and cell signalling [26, 27]. The Frizzled family is a recently identified group among the GPCRs, although its origin can be traced back to the arthropods. Ciona possesses a clear one-to-one ortholog each for human FZD3, FZD5 and FZD10 apart from a smoothened-like (SMOH) ortholog. The conserved (K-T-X-X-X-W) motif required for Wnt/β-Catenin signalling pathway can be observed two amino acids after the seventh transmembrane helix in all the recovered Ciona Frizzled receptors . An alignment of the TM regions of the Frizzled ortholog pairs (FZD) of humans and Ciona revealed that they are very well conserved with identities in range of ~48–60 %. The SMOH receptor shares ~57 % identity with its Ciona ortholog (ci0100150930) in the TM region. However, the Taste2 (TAS2) receptors seem to be absent in Ciona.
In a separate analysis, we identified four more Ciona Frizzled family members (ci0100152761, ci0100150136, ci0100138855 and ci0100153324) that were fragmentary models with less than 4 TM regions. These fragmentary sequences were not included in the final Ciona GPCR dataset for better handling of the MSA (Multiple sequence alignment) data for subsequent phylogenetic studies.
The Adhesionfamily (30 members)
The Ciona genome includes 30 Adhesion family members and is the second largest group of GPCRs after the Rhodopsin family. This family is characterized by long N-termini that contain multiple functional domains with adhesion-like motifs that are often sites of glycosylation events . It is noteworthy that Ciona with a relatively smaller genome possesses as many Adhesion members as any of the other larger vertebrate genomes [5, 6, 8]. The density (number of Adhesion genes/genome size) of GPCR Adhesion family is 1 gene/~5 Kb in Ciona whereas it is 1 gene/~90 Kb for human. This denser Ciona GPCR group relative to human genome is also accompanied by a lack of clear-cut homology to human Adhesion GPCRs. Among all the major family clusters in our analysis, the Adhesion family received the lowest bootstrap support (401 out of 1000 NJ trees) (Figure 2). Of the 30 Ciona receptors identified, 6 showed unambiguous orthologous relationship with human members in a large scale comparison of the Adhesion GPCRs of the two genomes (data not shown). Ciona Adhesion members that received complete support from its human counterparts were included in the final representation (Figure 2). The human genome contains 33 Adhesion receptors that display a phylogenetic clustering into eight main groups (I-VIII) . Ciona orthologs of the human Adhesion subfamilies includes one representative (ci0100145494) of the HE6/GP126 cluster (human group VIII), one representative (ci0100130008) of the GPR123-125 cluster (human group III) and one ortholog (ci0100132112) of the CELSR genes (human group IV) . The remaining 3 receptors (ci0100137028, ci0100140016 and ci0100150579) are orthologous to the LEC subfamily of human Adhesion group I.
A search for potential orthologs of the remaining 24 Ciona receptors using PIPEALIGN and further phylogenetic analysis with top hits from the search failed to identify unambiguous orthologs when performing both NJ and Maximum Likelihood analysis. Adhesion GPCRs that could not be assigned specific subfamilies due to lack of support in cross-genome phylogenetic analysis were deemed as "Unclassified Adhesion". 21 of the 24Ciona "Unclassified Adhesion" models had only the GPS proteolytic domain in the N-termini. Among the remaining three "Unclassified Adhesion" receptors, ci0100130017 had a LRR domain while ci0100132869 and ci0100131580 protein models possess EGF repeats in the N-termini.
A phylogenetic analysis of the Ciona Adhesion GPCRs was performed on the region from the TM 1 to TM 7 of the receptors. The analysis revealed that the Ciona Adhesion family clustered into five different groups each receiving more than 75 % bootstrap support in (1000 bootstraps) NJ trees (Figure 5). The five clusters were denoted as Group I to Group V. This group assignment of the Adhesion GPCRs is Ciona specific and is independent of the human Adhesion groups referred to by Bjarnadottir et.al., 2004 . 16 Ciona Adhesion receptors could be included into these five groups reflecting a probable paralogous relationship between the groups. The remaining Adhesion receptors remain as outgroups and do not cluster with sufficient phylogenetic support with other Ciona members. The phylogenetic analysis indicates that Ciona Adhesion repertoire constitutes a very diverse set of receptors sharing relatively poor sequence identity (Figure 5). The three members in Group III share the least identity (20%) while the two members in Group V were the most identical (89%). The Group V receptors are presumably gene duplication events as evident from the high bootstrap support (100%) of that node. Three members belonging to Group II share 25 % identity, while the five members of the Group I and the three members in Group IV share 23 % and 25 % identities respectively. In all cases, only the identities within the TM regions were considered. Group IV constitutes receptors that are orthologous to human LEC/ETL subfamily of receptors. In addition to the gene pair (ci0100152766/ci0100130804) in Group V, our analysis also lends support to a hypothesis of two more probable gene duplication events for the tree nodes (ci0100130776/ci0100139268) and (ci0100134612/ci0100152008) (Figure 5). Sequence alignment of the TM regions revealed that the ci0100130776/ci0100139268 pair receptors were 57% identical while the ci0100134612/ci0100152008 pair shares an identity of 52 %.
Specialized tunicate GPCRs
Our analysis revealed that the Ciona genome contains at least two representatives that could not be classified as unambiguous orthologs of the GRAFS families. These include one receptor (ci0100132129) that is similar to the protist Dictyostelium cAMP/Crl GPCR family and while the other receptor (ci0100150391) is homologous to the insect related Methuselah protein. The two Ciona receptors cluster with the corresponding cAMP and Methuselah GPCRs in a cross-genome phylogenetic analysis with a strong bootstrap support of 769 and 739 respectively, (out of 1000 replicates) in NJ trees (Figure 2). cAMP receptors in Dictyostelium discoideum mediate the coordinated aggregation of individual cells into a multi-cellular organism and regulate the expression levels of a number of developmentally regulated genes [30–32] while Crl receptors are implicated in cell growth regulation and tip formation in developing aggregates . The Methuselah GPCR in Drosophila plays a role in ageing and increased resistance to several forms of stress including heat, starvation and oxidative damage . The cAMP/Crl receptors are believed to be represented exclusively in protists, plant and fungal kingdoms while the Methuselah GPCR is thought to be present only in insects.
A BLASTP search for closest homolog of the Ciona cAMP GPCR-like sequence in the non-redundant NCBI database revealed that the Ciona sequence is most identical to predicted proteins in sea anemone (Nematostella vectensis) [Swiss-Prot: A7SN55] and Danio rerio [GenBank: XP_001332705] with an overall sequence identity of 42% for both cases. The recovered Nematostella sequence was observed to have only 5 TM regions. An alignment of the Ciona receptor and its sea anemone homolog showed an identity of nearly 37% when only the TM regions were considered. Among the Dictyostelium cAMP/Crl family members, the Ciona sequence ci0100132129 shows best identity with CARC (~21% identical in the TM regions), while the best identity among the Crl members is CrlA (~19 % identical in TM regions). Likewise, a BLASTP search with the Ciona Methuselah-like homolog (ci0100150391) with other insect genomes revealed that it is ~23% identical in the TM region to a MTH 10-like receptor in the Apis mellifera genome (PIR:UPI0000DB6BC5). However, the ectodomain that characterizes the Drosophila Methuselah GPCR is absent in the Ciona homolog.
Our phylogenetic analysis includes a collection of 169 putative Ciona GPCR sequences and highlights in detail the remarkable level of orthology between the protochordate and vertebrate GPCRs. Our analysis clearly suggests that a majority of the identified Ciona GPCRs have a clear vertebrate and in particular, a human homolog at the level of the major GRAFS subfamilies. Our results also show that at the individual gene level there are as many as 66 clear orthologs of human GPCRs in Ciona (Additional file 5). Given that Ciona possesses ancestral GPCR versions of the higher vertebral counterparts, there are also remarkable instances of lineage specific evolution as seen in the Rhodopsin and Adhesion families. From a protochordate perspective, the major differences from the human GPCRs in the Ciona repertoire can be attributed to the presence of 23 "Other Rhodopsins", the Ciona specific gene innovations of the LDLRR-GPCR cluster (14 sequences) and the unusually large Adhesion family (24 Unclassified Adhesion sequences). The presence of many "Unclassified/Other" GPCRs for which reliable orthologs could not be identified (via phylogenetic analysis) in other species, indicate that these are most likely ascidian specific genes. The other possibility is that these genes are evolving at a rapid rate, so much so that reliable orthologs could not be detected. In our final analysis and depiction of phylogenetic trees, we chose not to include these sets of "Unclassified/Other" sequences to avoid artefacts that can arise out of including sequences that are either evolving at a rapid rate or for which intermediate homologs could not be identified. Instances of incomplete/poorly modelled genes can also be a cause for the failure to detect reliable homologs for many of these GPCRs. With further sequencing and analysis of GPCRs in other protochordate genomes, it is likely to become clear if these genes are indeed protochordate related diversifications.
Our analysis of the Ciona GPCR family suggests that while the number of Adhesion receptors are comparable to that in vertebrates, the other GPCR families/subfamilies excepting that of the LDLRR-GPCRs, are represented at nearly 2 to 5 fold fewer numbers compared to the humans [Table 1, Table 2]. This observation is only expected for a genome that is 20 times smaller than the human genome.
Among the Rhodopsin receptors, the human α- and β-group subfamilies are analogously represented in Ciona while among the seven subfamilies of the human γ- – and δ-groups, orthologs of the PUR, classical CHEM, MCHR and MRG receptor subfamilies could not be identified by phylogeny (Additional file 6). Simple BLAST searches identified many Ciona receptors as being similar to chemokine receptor cluster, but they did not receive sufficient support in the phylogenetic analysis. These were annotated as "Unclassifed Chemokine receptor cluster-like". It is possible that these are highly divergent homologs of the chemokine receptor cluster genes. The absence of a clear classical chemokine (CCRs, CXCRs) receptor ortholog in Ciona agrees with previous finding that the evolution of chemokine receptors arose after the split of the vertebrates from the protochordate lineage .
Ciona has two representatives belonging to the prostaglandin receptor cluster. Although Drosophila has one remote homolog of a prostaglandin receptor, a readily recognizable homolog of a vertebrate-like prostaglandin is first observed only in the protochordate lineage. The sequence identities of Ciona prostaglandin receptors with human PTGER4 range from ~21 – 23%. Prostaglandin receptors mediate a wide variety of actions and play important physiological roles in the cardiovascular and immune systems and in pain sensation in peripheral systems . Reliable ESTs can be recovered from neural tissues for the PTGER homolog (ci0100153844) suggesting that these receptor homologs are involved in neural tissue physiology [Additional data file 2: sheet 2].
Among the peptide receptors, Ciona has a single copy of an authentic Tachykinin receptor which has been proposed to play an important role in feeding and sexual behaviour of the ascidians . Furthermore, putative receptors for peptides like GnRH, Cionin (CCK/gastrin-related peptide), Oxytocin/Arginine-vasopressin and Hypocretin/Orexin were recovered in Ciona [Additional data file 2]. Among the Ciona GPCRs the GnRH receptor remains the best studied and is the only known example of an authentic protochordate gonadotropin receptor to date . A GnRHR- like protein in Drosophila was later found to be activated by AKH, a peptide structurally similar to GnRH, thus making Ciona the only available protochordate model to possess authentic GnRHRs . So far, three GnRHR-like Ciona homologs have been found to be active in assays and are known to stimulate both the cAMP and IP3 signalling pathways. A cannabinoid receptor homolog (ci0100149095) and a receptor (ci0100136887) that is most closely related to the lysophospholipid receptors were also identified. We could not recover cannabinoid receptor homologs from the genomes of protostomes, while homologs were found in echinoderms suggesting that these receptors have their origins in a common deuterostomal ancestor to echinoderms and chordates. ESTs for the EDG homolog (ci0100136887) were recovered from blood cells/haemocytes suggesting that these receptors may have a role in the nervous system similar to that observed for vertebrate EDG receptors [40, 41].
Structural Innovations in the Ciona LDLRR/LGR-like/GLHR Rhodopsincluster
Ciona shows a notable lineage specific gene innovation and gene expansion (14 confirmed members) of the LDLRR-GPCRs (Low density lipoprotein receptor repeat containing receptors). These receptors have TM regions that are homologous to vertebrate INSL3/Relaxin binding GPCRs. Lineage specific gene expansions have long been observed in several cases including that of the opsin and fish odorant receptor expansions in puffer fish  and trace amine receptor expansion in zebrafish . The LDLRR-GPCR subfamily in Ciona displays an unusual combination of LDL-A domain tandem repeats in the N-termini. Although the presence of a single LDL-A domain in GPCRs was first observed in mammals, our unpublished study shows that the LDL-A domain repeats in GPCRs exist in protostomes (Molluscs, Arthropods) and in early deuterostomes like echinoderms (Strongylocentratus purpuratus) and Ciona suggesting that the origin of these receptors can be traced back to a common bilateral ancestor. The presence of LDL-A repeats in mosaic proteins has long been known and is an example that supports the exon shuffling theory [43, 44].
Based on phylogeny and domain architecture analysis we propose that while the signatures for the LDL-A domain and LRR domain containing Rhodopsin subfamily first arose in a bilateral metazoan ancestor, this subfamily has undergone several lineage specific innovations at the level of the combinations and tandem repeats of these domains. Our analysis suggests that the 14 Ciona LDLRR-GPCRs (in which the LRR domains are absent) have evolved divergently as a group [Figure 4, Additional data file 3]. This divergence is evident in the phylogenetic analysis of the TM regions of this group with those of the snail and human LGRs (Figure 4). It is possible that changes in the TM regions presumably accommodates for the changes that have occurred in the Ciona primary ligand binding N-terminal region that contain LDL-A domains but lack LRRs.
In vertebrate INSL3/relaxin binding GPCRs, a combination of a single cysteine-rich LDL-A domain and multiple LRRs at the ectodomain site is necessary for ligand binding, receptor activation and further downstream signalling from these receptors . Receptors that lack the LDL-A domain were found to bind relaxin, but were unable to effect cAMP accumulation . The LRR region in the ectodomain is essential for binding of glycoprotein hormones . Notwithstanding the incomplete sequence information in the case of the 14 "Unclassified LGR-like" sequences that do not have any identifiable domains at the N-termini, it is evident that the architecture of the 14 confirmed Ciona LDLRR-GPCRs that lack LRR domains is very different from vertebrate type C LGRs (LGR7/LGR8). These receptors are thus functionally unrelated to vertebrate INSL3/relaxin peptide binding receptors.
A typical cysteine-rich LDL-A domain found in human LDL receptor and human LDL Receptor related Protein (LRP) consists of ~40 amino acids containing six cysteine residues at fixed positions with the region between the fifth and sixth cysteines rich in aspartic and glutamic acid residues. These acidic residues in LDL receptors are known to interact with basic amino acids in lipoproteins [48, 49]. The human LDL receptor contains at least 7 class A repeats [50, 51] while the LRPs contain clusters of 2, 8, 10 and 11 repeats . Furthermore, in the LRPs, these repeats bind to large protein complexes like the α2 macroglobulin-protease complexes and urokinase-type plasminogen activator-plasminogen activator inhibitor (uPA-PAI) [53, 54]. A majority of LDL-A-like repeats in the 14 Ciona LDLRR-GPCRs conform to the classical LDL-A repeat structure. It is thus tempting to speculate that these domains are crucial for the specific function of the receptors and that these receptors likely bind to lipoproteins or large protein complexes as ligands, analogous to the classical human LDL receptor. This hypothesis based on comparative domain analysis may serve as a pointer towards further molecular studies. A similar hypothesis that the snail GRL101 receptor which contains both LDL-A and LRR repeats might be a member of a putative class of GPCRs that directly transduce signals carried by large extracellular (lipo)protein (complexe)s, has been proposed earlier . The differences between the snail LGR-like and the Ciona LDLRR-GPCRs lie in the possibility that the snail receptor which contains LRRs can potentially offer a different ligand presentation mechanism compared to the Ciona receptors that lack LRRs.
Although direct evidence of lipoproteins as GPCR signalling agents is not available, previous evidence suggests that LDL molecules at physiological concentrations can activate phosphatidylinositol signalling and mobilization of calcium from intracellular stores in the absence of LDL receptor mediation [55, 56]. Similarly, HDL stimulation of cAMP formation has also been demonstrated . However, to the best of our knowledge, no authentic GPCR has been demonstrated to bind to lipoproteins as signalling molecules and therefore it should be a crucial step forward to de-orphanize this family of GPCRs in Ciona. A search of the Ciona EST database for matches to the novel Ciona LDLRR-GPCR protein models revealed that a majority of the hits are derived from the blood tissue and haemocytes [Additional data file 2: sheet 2]. Identification of three putative Ciona orthologs of vertebrate INSL-relaxin-like genes has recently been reported . Furthermore, the authors hypothesize that the Ciona INSL/RLN orthologs most likely interacts with a Ciona RTK-receptor and not a GPCR and the receptor switch from RTK-receptor to a GPCR occurred after the split of Ciona from the common lineage leading to the vertebrates . From our own analysis of the 32 Ciona receptors in the LDLRR-GPCR/LGR-like/GLHR cluster, we found two LRR bearing LGR-like sequences (ci0100148288 & ci0100151424). Conserved domain searches of these two receptors further narrowed down only one candidate INSL3/relaxin receptor-like (ci0100148288) sequence. A phylogenetic analysis of Ciona sequence (ci0100148288) with vertebrate type C INSL3/Relaxin receptors (Figure 4) revealed that the Ciona receptor is not orthologous to vertebrate type C LGRs and is therefore unlikely to bind to vertebrate INSL3/Relaxin versions. Our analysis does not however exclude the possibility that the two LGR-like Ciona sequences (ci0100148288 & ci0100151424) can bind to the Ciona INSL-relaxin orthologs.
The CionaGPCR Adhesome
Unlike vertebrates which possess multiple functional Adhesion N-terminal domains that include lectin, cadherin and laminin domains among others, the Ciona GPCR adhesome possesses a less complex domain organisation with a single GPS domain in 21 out of 30 receptors. Furthermore, these receptors did not correspond with any clear ortholog in other vertebrates. Orthologs for the vertebrate CELSR, LEC, GP123-GP125 and HE6 class of receptors could be observed but orthologs of other Adhesion members previously documented in human are missing.
The ligands and biological functions of Adhesion GPCRs are largely unknown. Orthologs belonging to the CELSR, HE6 and GP123/124/125 subfamilies have only a single representative in Ciona making it an interesting model for studying the roles of these GPCRs. The CELSR family of GPCRs in mammals play a possible functional role in the developmental processes of the brain and the peripheral nervous system . Although a search of the Ciona EST database did not reveal any analogous distribution of CELSR homologs in neural tissues, this does not necessarily exclude its expression in these regions. The Ciona CELSR model showed maximum (~34%) identity with human CELSR2 while displaying a remarkable level of conservation in the N-terminal domain architecture. The Ciona CELSR homolog contains 9 cadherin domain repeats, 2 laminin G-like motifs, 1 laminin EGF-like motif, 7 EGF-like calcium binding motifs and the characteristic GPS domain identical to that in human CELSR2.
Among mammalian LEC receptors, LEC1 and LEC3 expression is abundant in the brain tissue . LEC1 binds to α-latrotoxin and has a role in exocytosis of the synaptic vesicles . The other vertebrate LEC homologs are unable to function as a-latrotoxin receptors and their physiological roles remain unknown. Ciona also has at least three phylogenetically identifiable LEC homologs for which reliable ESTs can be recovered from the gastrula and neurula, cleavage stage embryo and tail-bud embryo developmental stages suggestive of a role for these receptors in developmental processes [Additional data file 2: Sheet 2]. It should be interesting to explore the presence of LEC homologs in neural tissues of Ciona to compare biochemical roles of LEC GPCR biology between vertebrates and protochordates. As many as three gene duplication events can be suspected in the Ciona GPCR adhesome (Figure 5). Ciona can be a very good model where the developmental role of Adhesion orthologs can be investigated at the single cell level because the larvae contain approximately 2600 cells and the lineage of the embryonic cells giving rise to different tissue types has been well determined .
The cAMP GPCR-like and Methuselah-like GPCR in Ciona
The cAMP/Crl GPCRs belong to an ancient family of GPCRs with homologs observed in the protists, fungi and plants as reported in the Pfam database . However, authentic cAMP GPCRs have been characterized only in the protist Dictyotelium discoideum . The Ciona cAMP GPCR-like sequence shows best identity with predicted hypothetical proteins in Danio rerio and Nematostella vectensis. The top hit in a search with the Ciona cAMP GPCR-like sequence (ci0100132129) in the PRINTS database was the Dictyostelium cAMP receptors (CARs) with matches for 4 out of 7 sequence motifs and an E-value of 1.6e-05 . The next best hit was the GABAB GPCR of the Glutamate family with matches for 2 out of 14 sequence motifs and an E-value of 23. A recent study on the GPCR repertoire in the Branchiostoma floridae genome reports two cAMP GPCR-like sequences with limited sequence identities . The ESTs for the Methuselah-like sequence (ci0100150391) were found only in the adult digestive gland suggesting a possible role for this GPCR in digestive processes. [Additional data file 2: sheet 2].
Although the putative non-GRAFS Ciona GPCRs ci0100132129 and ci0100150391 cluster with cAMP and Methuselah GPCR families with significant bootstrap support, they share only limited sequence identities (~19–25 %) with homologous members of their respective families. Evidently, sequence homology at these levels does not imply functional homology. It is possible that these sequences are highly divergent homologs of the cAMP and Methuselah GPCR families. The recovery of Ciona ESTs for both receptors confirmed that these genes are endogenously expressed. [Additional data file 2: Sheet 2].
GPCR Orthologs of human disease genes
Ciona has representatives of GPCRs that are associated with diseases in humans. Among the 15 monogenic GPCR disease genes known to date, Ciona has orthologs of 6 receptors [Table 3]. The motivation for identification of these GPCR orthologs is to facilitate the conception of studies that will have implications for human health. For example, the mammalian CASR gene is known to be expressed in parathyroid glands and its loss in parathyroid gland in mice is implicated in hypercalcemia, hyperparathyroidism and growth retardation . Similarly, monogenic mutations of the PTHR1 gene is known to be involved in chondrodysplasia and multi-organ disorders, while monogenic mutants of Vasopressin receptor (AVPR2) are implicated in nephrogenic diabetes insipidus . EST matches for CASR, PTHR1 and AVPR2 orthologs can be recovered in Ciona [Additional data file 2: Sheet 2]. The tunicate can thus prove an excellent model to probe the roles of these genes. Although physiological roles of these receptors may differ between humans and Ciona, studying their roles at the biochemical and genetic level in Ciona will potentially pave the way for a better understanding of its vertebrate counterparts.
Sequence conservation of CionaPTHRs
A particular instance of orthology that will provide clues for the physiological roles of ancestral GPCRs is the PTHR orthologs in Ciona that share a high sequence identity with both vertebrate PTHR-1 and PTHR-2. PTHR-1-ligand interactions and their mechanism of action in vertebrates are well understood. PTHR-1 is abundantly expressed in bones and kidney and is known to play an important role in mediating PTH regulated mineral ion homeostasis and endochondral bone formation . Mutations in vertebrate PTHRs and ligands are linked to a number of genetic diseases affecting skeletal development and calcium homeostasis . Hence, the discovery of homologs for these receptors in a protochordate system is of special interest. Unlike PTHR-1, which is ubiquitously expressed, PTHR-2 is expressed in brain, testis, placenta and cardiac endothelium. The physiological roles of PTHR-2 however, are not clearly established .
A multiple sequence alignment of Ciona PTHRs with those of vertebrates reveal several conserved motifs [Additional data file 4]. The sequence conservation across many functionally important residues responsible for ligand binding and downstream G-protein coupling makes it likely that the putative Ciona PTHRs can cross-react specifically with mammalian PTH/PTH related peptides and can signal downstream by coupling to G-proteins. Interestingly, Ciona PTHR homologs exhibit high sequence conservation across sites known to be associated with Blomstrand's [70–72] and Jansen's syndrome in humans [73–75]. The absence of defined N- and C-terminal regions in Ciona PTHR homologs again seem to be a result of incomplete modelling and a clear picture should emerge with further refinement of the genome or through experimental studies. Information from the existing protein models (ci0100139945 and ci0100151327) should prove useful for performing experiments addressing the physiological roles of these receptors in a protochordate and its evolution in subsequent lineages.
A genome-wide analysis of the repertoire of putative GPCRs in Ciona intestinalis was carried out, revealing many intriguing aspects of ascidian GPCR evolution. While as many as 39% of Ciona GPCR sequences are orthologous to vertebrate GPCR subfamilies, a substantial number seem to represent invertebrate-chordate related diversification. The observation of orthologs of several GPCRs related to vertebrate heart and neural tissue physiology raises the possibility that genetic studies of Ciona GPCRs can be exploited to assess the functions of complementary higher vertebrate genes. The availability of Ciona EST hits with matches for 133 of the 169 putative GPCRs will facilitate further studies to elucidate their functional roles. The compact GPCR repertoire in the Ciona model also presents fewer complications in terms of functional redundancy and offers an advantage to study the evolution and function of these receptors. In summary, our analysis leads the way towards understanding the invertebrate-chordate GPCR signalling system and helps identify candidate vertebrate GPCR homologs in Ciona to be selected for functional studies.
Description of Ciona intestinalissequence data set
The official version of the Ciona intestinalis JGI v2.0 database was used as the source for obtaining the complete proteome . The current size of this tunicate assembly is 173 Mb with approximately 94 Mb of the assembly being mapped to chromosomes, while the remaining 45 % of the genome is included in scaffolds. The proteome dataset includes 15,852 proteins and sequences in the current version include both automated and to a lesser extent, manually curated annotations. A CD-HIT  was performed on the proteome set with 99% identity cut-off to obtain a non-redundant dataset that is devoid of splice variants, duplicates and polymorphisms. Non-redundancy of the data set was also verified by a manual check.
Prediction of Transmembrane regions
Transmembrane regions in the Ciona proteome were predicted using secondary structure predictions tools like HMMTOP , TMHMM  and SOSUI  with default settings. A majority of known GPCRs acquire maximum coverage in the 7 TM domain prediction analyses . Since the default settings have been known to under- predict or over-predict TM segments, peptides covering the entire range of 6, 7 and 8 TM domains were retrieved using each of the above programs. The predicted sequences recovered from these programs were later clustered and any redundancy in the (6–8) TM data set was removed. This resulted in 645 unique putative GPCR sequences that were analyzed for GPCR specific patterns using a variety of similarity and pattern search tools in a concerted and comprehensive manner. The strategy to identify protein models with 6/7/8 transmembrane regions while missing out on fragmentary or incomplete GPCR models from the JGI Ciona proteome allows for a better handling of the putative GPCR data set for multiple sequence alignment and phylogenetic studies.
Identification of GPCRs using HMMPFAM
Customized HMM models for all known GPCR families/subfamilies recognized by the GPCRDB , were constructed from ClustalW version 1.83  generated multiple alignments using HMMBUILD and HMMCALIBRATE programs of HMMER package version 2.3.2 . HMMPFAM program was used to query a database of custom-built GPCR HMMs and all (6–8) TM Ciona queries that returned hits with an E-value better than 0.01 were extracted. The HMM files and seed alignments are available upon request. Default settings were used in all HMMBUILD and HMMCALIBRATE model builds.
Identification of GPCRs using RPS-BLAST
Position Specific Scoring Matrices (PSSMs) for all GPCR families/subfamilies recognized by the GPCRDB were generated using BLASTPGP program . A collection of these GPCR PSSMs was then formatted using FORMATRPSDB to construct RPS-BLAST databases. All GPCR family specific PSSMs were generated with known GPCR sequences being used in a two-round iteration search against the corresponding GPCR family-specific database obtained from GPCRDB. For example, a CLASS A (Rhodopsin) Amine family PSSM is generated by BLASTPGP using a set of known CLASS A Amine GPCRs as a query against a CLASS A amine database with two rounds of iteration (-j option). FORMATRPSDB operations were carried out with default settings. GPCR family or subfamily specific PSSMs are available upon request. The Ciona (6–8) TM data set was searched against a database of custom-made GPCR PSSMs by an RPS-BLAST search with a cut-off at E = 0.001 and the best hits were recovered.
Identification of GPCRs using BLASTP
GPCR sequences were downloaded from the ftp site of GPCRDB and BLASTP  similarity comparisons were performed using the Ciona (6–8) TM dataset as query with an E-value = 10-12. Ciona queries that returned hits better than the cutoff were extracted into a temporary file.
Identification of remote homologs
In order to identify homologs for receptors that did not identify themselves with any human sequence, we performed PIPEALIGN searches. PIPEALIGN  is a web service that enables collection of potentially homologous sequences based on the query through a series of automated and integrative profile based search steps and finally presents the retrieved hits clustered into subfamilies in the context of a multiple sequence alignment (MSA). The MSA cluster results from PIPEALIGN proved to be a good starting point from which closest neighbours could be identified with a quick-fire NJ or ML analysis. The nearest neighbour (the species adjacent to the query through the least number of internal nodes or in the case of a tie, via the shortest branch length) from such an analysis was identified and hand picked for inclusion into the final analysis.
For example, a typical identification of Ciona cAMP GPCR-like and Methuselah-like receptors was carried out by performing searches of these sequence queries on the web-versions of PIPEALIGN, Interproscan  and BLASTP (Blastp of Uniref100 database ). The hits from these searches were used for a preliminary NJ and ML analysis. After a manual check of the alignment for the hits, the nearest neighbour from such an analysis was then included into the final representative phylogenetic tree reconstruction.
Orthology assignment using BLASTP and phylogenetic methods
Human GPCR sequences were obtained from Fredriksson et.al  as well as from the GPCRDB. Ciona protein models that were returned as hits based on similarity/pattern searches (HMM/BLASTP/RPS-BLAST) were compiled together into one dataset. This initial dataset includes many redundant sequences identified simultaneously by two or more similarity/pattern searches. The final non-redundant Ciona GPCR dataset consisted of 169 proteins.
To ensure that orthology assignments are sound, a two-way BLASTP search was initially performed followed by phylogenetic analysis. Both Ciona-human and human-Ciona BLASTP searches were carried out resulting in the identification of sequences that were the top hits to each other. Orthologs were also detected based on cross-genome phylogenetic analysis of the human-Ciona GPCR datasets. The final orthology assignment was arrived at based on a consensus of the BLASTP/Reverse BLASTP results and the cross-genome phylogenetic analysis [Additional data file 2: sheet 1]. This final orthology assignment was also independently verified by searches of the Ciona sequences against conserved domain databases (Interproscan) and stand-alone local GPCR HMM databases.
Identification of genomic positions of GPCRs using TBLASTN
The best Ciona genome positions were identified for each Ciona GPCR using TBLASTN with an E-value cut off at 10-5. All hits were manually inspected and the best genomic positions providing entire coverage of each query was extracted and imported in Excel sheets. [Additional data file 2: sheet 1]
EST-hits and tissue/developmental stage based expression data
The entire UniGene database of Ciona was downloaded from the ftp site  and locally installed. Ciona GPCR sequences were queried against the UniGene database using TBLASTN with the E-value set at 1e-15. The identifiers of the EST-hit collection were imported in an excel sheet and categorized based on their derivative developmental stage/tissue source to assist in gene finding and transcriptome analysis. [Additional data file 2: sheet 2]
Based on the initial Ciona-human BLASTP searches, Ciona GPCRs were broadly divided into Rhodopsin families and subfamilies and non-Rhodopsin receptors. The broadly separated GPCR families were then combined with their related GPCRs from either human or other species. MSAs were then performed using MAFFT version 5  with protein weight matrix JTT200 and employing the E-INS-I strategy with a gap opening penalty of 1.53 and offset value of 0.123. All phylogenetic analyses shown arise out of consideration of only the terminally truncated TM spanning regions. Deviant branches were analysed and the corresponding sequences checked for false positives by looking for GPCR specific signatures or through Interproscan searches. The GRAFS (Glutamate, Rhodopsin, Adhesion, Frizzled, Secretin) dataset, the GPCR adhesome, the cAMP GPCR analysis and the non-(LDLRR-GPCR/LGR-like) Rhodopsin subsets were bootstrapped 1000 times using SEQBOOT from the PHYLIP package [version] . The alignments were then used for subsequent distance matrices (JTT matrix) calculation by PROTDIST to obtain 1000 matrices. To obtain unrooted trees, neighbour-joining method was employed (NEIGHBOR) and a consensus of 1000 neighbour trees was arrived at using CONSENSE. The topology and phylogenetic support for the above mentioned tree representations was also verified using TREE-PUZZLE analysis. The divergent "Unclassified/Other" GPCRs were removed from the final data set to avoid tree artefacts .
Maximum likelihood tree of the LGR-like/LDLRR-GPCR cluster was obtained after performing 10,000 quartet puzzling steps with TREE-PUZZLE [version 5.2]  using a percentage of invariant sites to be estimated and eight rate categories to describe among-site rate variation with the shape parameter estimated from the data and employing VT (Mueller-Vingron 2000) substitution model. The NJ trees were visualized and edited using SPLITSTREE version 4  and the TREE-PUZZLE trees were visualized using Tree view 
Brain Angiogenesis Inhibitor
Calcium Sensing Receptor
Calcitonin gene related peptide Receptor
Cadherin EGF LAG Seven G-type Receptors
Corticotropin Releasing Hormone Receptors
Endothelial Differentiation GPCR
Expressed sequence tag
Epidermal Growth Factor
EGF-Latrophilin 7 TM domain
Glycoprotein hormone Receptor
G protein-coupled receptor
Glucagon-like peptide Receptor
Gonadotropin Releasing Hormone Receptor
Gastric Inhibitory Polypeptide Receptor
GPCR Proteolytic site domain
Hidden Markov Model
Low Density Lipoprotein Receptor ClassA domain
Low Density Lipoprotein Receptor repeats
Leucine Rich Repeat containing GPCR
LDL Receptor related Proteins
Leucine Rich Repeat domain
Lutenizing hormone/chorio-gonadotropin receptor
Lung 7TM Receptor
MAS1 oncogene receptor
Melanin Concentrating Hormone Receptor
Melanocortin, Endothelial Differentiation, Cannabinoid, Adenosine GPCR cluster
Multiple Sequence Alignment
Neighbour Joining tree reconstruction method
Pituitary Adenylyl Cyclase Activating Protein
Position Specific Scoring Matrix
Parathyroid Hormone Receptor
Retinoic acid inducible GPCR
Receptor Tyrosine Kinase
Somatostatin and Angiotensin-like peptide receptor
Somatostatin, Opioid, Galanin cluster
Terminally truncated transmembrane spanning segments
Very Large G-Protein-Coupled Receptor
Vasoactive intestinal peptide Receptor
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We thank the Joint Genome Institute (JGI) for public availability of sequencing data. These sequence data were produced by the US Department of Energy JGI (USA). We also thank IIT Madras and the Bioinformatics Infrastructure Facility, supported by the DBT, Govt of India, for infrastructural support.
NK carried out the work and has written the first draft of the paper. GKA participated in discussions of the study. NM conceived the study, participated in the analysis and coordination and helped drafting of the final manuscript.