Table 5 List of primers and edition of sequences
From: Multilocus analysis of introgression between two sand fly vectors of leishmaniasis
locus | primers | Removed gap sitesa | NR blocksb | Removed sequences from NR blocksc |
|---|---|---|---|---|
Ca1D | 5LWIca1D 5'-CAGGATATAATGATGGATTG-3' 3LWIca1D 5'-CACGAACAAGTTGATAAT-3' | 159–160; 162–169; 183 | 1–176 | IAFCa_A12; IAFCa_F09; WAFCa_B12; WAFCa_C09; WAFCa_H11; WAFCa_H12 |
Cac | 5Llcac 5'-GTGGCCGAACATAATGTTAG-3' 3Llcac 5'-CCACGAACAAGTTCAACATC-3' | 10; 19; 124–126; 129–132; 150; 170; 180; 182 | 122–172 | IAFcac_H05; WAFcac_F01; WAFcac_H07 |
Per | 5llper2 5'-AGCATCCTTTTGTAGCAAAC-3' 3llper2 5'-TCAGATGAACTCTTGCTGTC-3' | 154–157; 167–169; 204 | 125–210 | IAFPER16; WAFPER05 |
Rp49 | 5RP49semideg1 5'-TTCATYCGYCAYCAGWSBGA-3' 3llRP49exp2 5'-GGGCGATCTCAGCACAGTAT-3' | 29–36; 46–49; 55–56; 59; 65–69; 72; 74; 87 | 1–237 | IAFrp49_B08; IAFrp49_C08; IAFrp49_E05; IAFrp49_E08; IAFrp49_G08; IAFrp49_H06; WAFrp49_D11 |
RpL17A | 5LLrpL17A 5'-TCAATTGCGCCGACAATAC-3' 3LLrpL17A 5'-GCTGATCCTTTCATTTCGCC-3' | 90–91; 101–102; 109–110; 131; 135; 144; 147–148; 153–154 | 1–114 | IAFr17a_D10; IAFr17a_E10; IAFr17a_F10; IAFr17a_G11 |
RpL36 | 5LWIrpL36 5'-GTTCCTCACGCTTCCTCTTG-3' 3LWIrpL36 5'-AAAGTGAAAGGACTCCGCCC-3' | 26–29; 46; 52; 65; 77; 215–217; 226; 238–253; 267; 270; 274; 289–294; 362 | 126–238 | IAFr36_D07; IAFr36_H11 |
RpS19a | 5LWrpS19 5'-TGATCAACACAAGATTGTCCG-3' 3LWrpS19 5'-ACACCATTCCTCTTACGACC-3' | 174 | 77–193 | IAF19_G01; IAF19_H02; WAF19_B08; WAF19_F079 |
TfIIA-L | 5LLTfIIA-L 5'-GATAATGATCCAGACGATGCC-3' 3LLTfIIA-L 5'-GAAAACATAGTCCTTCCCACC-3' | 162; 173–179; 201–259; 277; 284–319; 341; 345–346; 369–370; 386–387 | 1–254 | - |
Up | 5LLup 5'-GCAACAAGTCCAAAGAGCAG-3' 3LLup 5'-TCATAGGAGCGGGTGTCAAC-3' | 189–191; 224; 351–355; 360–361; 368–369; 396–397 | 1–354 | - |
zetacop | 5LLzetacop 5'-GGATGCAGATCCTTCATCCG-3' 3LLzetacop 5'-CGACCACTTCAGTTGTTCTC-3' | 115; 131–134; 149–150; 168; 181; 195; 224; 234–235; 240–242 | 1–230 | IAFztp_H07; WAFztp_C06 |