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Table 2 Primer sequences.

From: Origins of asexuality in Bryobiamites (Acari: Tetranychidae)

Primer

Sequence 5' to 3'

Reference

Position

COI_F1

TGATTTTTTGGTCACCCAGAAG

[31]

2173

COI_F2

AAGAGGAGGAGGAGACCCAA

[77]

2133

COI_F3

WGTHTTAGCAGGAGCAATTACWAT

modified from [78,79]

2067

COI_F4

GGAGGATTTGGAAATTGATTAGTTCC

[80]

1693

COI_R1

TACAGCTCCTATAGATAAAAC

[31]

2605

COI_R2

AAWCCTCTAAAAATRGCRAATACRGC

modified from [77]

2620

28S_D1_F

ACCCSCTGAAYTTAAGCAT

[81]

 

28S_D1_R

AACTCTCTCMTTCARAGTTC

[81]

 
  1. Primers used for amplification and sequencing of part of the COI and 28S D1 region. The position of the COI primers on the COI gene is listed, position numbers correspond to the Drosophila melanogaster mitochondrial DNA sequence [GenBank:U37541]. The fragment between primers F1 and R1 (410 bp) was used for analyses in this study.