Figure 6

Expression analysis of the KCNQ1OT1 . Primers designed from intron 10 of KCNQ1 were used to determine expression of the KCNQ1OT1 anti-sense RNA. Primers yield a single 400 bp band as confirmed by genomic DNA PCR (result not shown). Expression was only detected in the trilaminar portion of the placenta (TYS) and not the bilaminar (BYS) and only in the final stages of pregnancy. RT+ denotes samples that have been reverse transcribed. RT- denotes DNased RNA, also used in the PCR reaction to ensure no DNA carryover. -VE represents the negative control reaction in which template was omitted and M, indicates DNA marker.