Genetic evidence from Indian red jungle fowl corroborates multiple domestication of modern day chicken

Table 1 Microsatellite loci and PCR conditions used in the present study, and population-wise number of alleles obtained for Indian chicken. Original references are listed in the table.

Locus	Repeat motif	Allele Size range	Tm °C	MgCl₂	Total no. of alleles*			Reference
					G. sonneratii	G. g. murghi	G. g. domesticus
ADL210	(AC)₁₅	116–130	46	1.5 mM	1	2	5	[41]
CALB1	(T)₂₅	76–106	55	2.5 mM	2	3	3	[42]
HSF3A	(GAG)₁₀	230–259	55	1 mM	2	12	8	[42]
HUJ1	(GT)₂₃	150–180	55	1.5 mM	4	11	7	[43]
MCW1	(GT)₉	157–171	62	1.5 mM	1	6	5	[42]
MCW305	(GT)₁₃	250–275	55	1.5 mM	3	3	7	[43]
MCW306	(AT)₁₁	142–176	55	1.5 mM	4	9	6	[43]
MCW4	(TG)₈	146–188	67	2.5 mM	2	15	3	[18]
MCW5	(TG)₆AA(TG)₆(A)₁₅ (GA)₂(GAA)₂₁	184–263	62	1.5 mM	4	18	9	[18]
MYCN	(TG)₁₇	174–210	69	2 mM	4	12	5	[43]
VITIIG2	(TTTG)₆	143–179	51	3 mM	5	15	1	[41]
Total					32	106	59

ISSN: 2730-7182