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Figure 3 | BMC Evolutionary Biology

Figure 3

From: The tiny Hairless protein from Apis mellifera: a potent antagonist of Notch signaling in Drosophila melanogaster

Figure 3

A.m.H interacts with D.m.H partner proteins. Protein-protein interactions were tested with the yeast two-hybrid assay. A.m.H wild type and mutant constructs are depicted schematically and were provided in pEG vector that contains the LexA-DNA binding domain. AmH 4-1, N-terminal truncation; A.m.HG*, GBD mutant; A.m.HC*, CBD mutant; A.m.HGC*, double mutant; GBD, Gro-binding domain only; GBD*, mutant construct. Dm-Su(H) and Dm-S4-1 were in pJG; Dm-Gro and Dm-CtBP in VP16 vectors. Empty vectors served as controls. Blue color denotes positive interactions as it reflects activation of the lacZ-reporter gene.

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