Figure 7
The acidic domain in D.m.H attenuates H repressor activity. A) Position of the acidic domain (AD) downstream of the SBD in D.m.H protein. Two deletion constructs (ΔAD, ΔC3) were generated lacking the indicated parts. B) Overexpression of full length D.m.H in the central domain of the wing disc (omb-Gal4>UAS-FLH) results in a truncated wing with a defective distal wing margin. C) In comparison, A.m.H overexpression causes a deep incision, deleting most of the wing blade. D) Overexpression of D.m.H lacking just the acidic domain (omb-Gal4>UAS-HΔAD) causes a much stronger phenotype: the wing is barely detectable, mainly the hinge region remains. E) A similar, albeit less extreme phenotype is observed when D.m.H without the C3 domain is overexpressed (omb-Gal4>UAS-HΔC3).