The molecular basis of color vision in colorful fish: Four Long Wave-Sensitive (LWS) opsins in guppies (Poecilia reticulata) are defined by amino acid substitutions at key functional sites

Table 2 Quantitative PCR data.

Individual	Transcript	Ct (dR)	Original Copy Number
Male	A180	24.35	6758
Male	S180	29.54	121
Male	S180r	33.12	39
Male	P180	39.01	1+
Female #1	A180	25.46	3126
Female #1	S180	30.10	78
Female #1	S180r	33.90	24
Female #1	P180	39.58	1+
Female #2	A180	24.49	6131
Female #2	S180	27.93	416
Female #2	S180r	32.36	63
Female #2	P180	37.51	1+

Ct (dR) values are shown for each LWS opsin transcript from three adult guppies. Original copy number was calculated using Ct (dR) values and the standard curve data shown in Table 3.

ISSN: 2730-7182