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Figure 1 | BMC Evolutionary Biology

Figure 1

From: ITS2 data corroborate a monophyletic chlorophycean DO-group (Sphaeropleales)

Figure 1

Flowchart of the methods applied in this study. Sequences were obtained from the laboratory and from NCBI and afterwards folded with RNAstructure [38] or custom modelling of the ITS2 Database [3033]. An alternative way may pose to directly access sequences and structures deposed at the ITS2 Database. The sequence-structure alignment was derived by 4SALE [40]. Afterwards several phylogenetic approaches were used to calculate trees: NJ = neighbour-joining, PNJ = profile neighbour-joining, strPNJ = sequence-structure neighbour-joining, prePNJ = predefined profiles profile neighbour-joining, MP = maximum Parsimony, ML = maximum likelihood and B = Bayesian analysis.

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