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Figure 5 | BMC Evolutionary Biology

Figure 5

From: Robustness promotes evolvability of thermotolerance in an RNA virus

Figure 5

Design for an evolution experiment where bacteriophage φ6 lineages were selected to withstand damaging effects of heat shock. A virus lysate was exposed to 45°C incubation for 5 minutes, and a dilution of the surviving progeny was plated on a lawn of P. phaseolicola bacteria. Overnight plaque formation at the standard temperature of 25°C corresponded to 5 generations of virus evolution. The plaques were then harvested to remove bacteria, and the process was repeated. Duration of the experiment was 10 days, equivalent to 50 virus generations.

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