Evidence for positive selection acting on microcystin synthetase adenylation domains in three cyanobacterial genera

Tooming-Klunderud, Ave; Fewer, David P; Rohrlack, Thomas; Jokela, Jouni; Rouhiainen, Leo; Sivonen, Kaarina; Kristensen, Tom; Jakobsen, Kjetill S

doi:10.1186/1471-2148-8-256

Table 1 Strains compared in present study

From: Evidence for positive selection acting on microcystin synthetase adenylation domains in three cyanobacterial genera

Strain^#	Geographic origin	Year	Genes^&	Microcystin isoforms produced [reference]
			mcyB* mcyC
Anabaena
N-C 83/1	L. Edlandsvatnet, Norway	1981	EU009900 EU009918	[D-Asp³]MC-LR, [D-Asp³]MC-RR, MC-LR, MC-RR	[56]
N-C 267/4	L. Fammestadtjønni, Norway	1990	EU009901 EU009919	MC-HtyR, MC-LR, MC-FR, [D-Asp³]MC-LR, [D-Asp³]MC-HtyR, [D-Asp³]MC-FR, MC-HilR, MC-HphR	[56]
N-C 269/2	L. Frøylandsvatnet, Norway	1990	EU009902 EU009920	[D-Asp³]MC-LR, MC-HtyR, [D-Asp³]MC-HtyR, MC-LR, [D-Asp³]MC-FR, MC-FR, MC-HilR, MC-HphR, [D-Asp³]MC-HilR, [D-Asp³]MC-HphR	[56]
N-C 269/6	L. Frøylandsvatnet, Norway	1990	EU009903 EU009921	[D-Asp³]MC-LR, MC-HtyR, [D-Asp³]MC-HtyR, MC-LR, [D-Asp³]MC-FR, MC-FR, MC-HilR, MC-HphR, [D-Asp³]MC-HilR, [D-Asp³]MC-HphR	[56]
N-C 270/1	L. Arefjordsvatnet, Norway	1990	EU009904 EU009933	[D-Asp³]MC-LR, MC-LR, [D-Asp³]MC-RR, MC-RR	[56]
90	L. Vesijärvi, Finland	1986	AJ536156 AJ536156	MC-LR, [D-Asp³]MC-LR, MC-RR, [D-Asp³]MC-RR, MC-HilR, [D-Asp³]MC-HilR	[9]
1TU44S16	L. Tuusulanjärvi, Finland	2001	EU009887 EU009905	[D-Asp³]MC-LR, MC-LR	[56]
1TU30S4	L. Tuusulanjärvi, Finland	2001	EU009888 EU009906	[Dha⁷]MC-LR, [D-Asp³, Dha⁷]MC-LR, [L-Ser⁷]MC-LR	[56]
1TU31S9	L. Tuusulanjärvi, Finland	2001	EU009889 EU009907	[Dha⁷]MC-LR, [D-Asp³, Dha⁷]MC-LR, [L-Ser⁷]MC-LR, [D-Asp³, demet-N⁷]MC	[56]
202A1/35	L. Vesijärvi, Finland	1987	EU009890 EU009908	[D-Asp³, Dha⁷]MC-LR, [Dha⁷]MC-LR, [L-Ser⁷]MC-LR	[56]
1TU46S11	L. Tuusulanjärvi, Finland	2001	EU009891 EU009909	[D-Asp³]MC-LR, MC-LR, [D-Asp³]MC-HilR	[56]
202A2/41	L. Vesijärvi, Finland	1987	EU009892 EU009910	[D-Asp³, Dha⁷]MC-LR, [Dha⁷]MC-LR, [L-Ser⁷]MC-LR, [D-Asp³, demet-N⁷]MC	[56]
0TU33S16	L. Tuusulanjärvi, Finland	2000	EU009893 EU009911	[D-Asp³]MC-LR, MC-LR, [D-Asp³]MC-HilR	[56]
258	L. Tuusulanjärvi, Finland	1990	EU009894 EU009912	MC-LR, [D-Asp³]MC-LR, MC-HilR, [D-Asp³]MC-HilR	[56]
1TU32S11	L. Tuusulanjärvi, Finland	2001	EU009895 EU009913	[Dha⁷]MC-LR, [D-Asp³, Dha⁷]MC-LR, [L-Ser⁷]MC-LR	[56]
BIR 246	Gulf of Finland, Baltic Sea	2004	EU009896 EU009914	[D-Asp³]MC-HtyR, MC-HtyR, [D-Asp³]MC-LR, MC-LR, [D-Asp³]MC-FR, MC-FR, MC-HphR, [D-Asp³]MC-HphR, MC-HilR, D-Asp³]MC-HilR	[56]
288	Littoisten vesilaitos, Finland	1990	EU009897 EU009915	MC-HtyR, MC-LR, MC-FR, [D-Asp³]MC-LR, [D-Asp³]MC-HtyR, MC-HphR, [D-Asp³]MC-FR,	[56]
315	Gulf of Finland, Baltic Sea	1997	EU009898 EU009916	[Dha⁷]MC-HtyR, [D-Asp³, Dha⁷]MC-HtyR, [Dha⁷]MC-LR	[56]
318	Gulf of Finland, Baltic Sea	1998	EU009899 EU009917	MC-HtyR, [D-Asp³]MC-Hty, [D-Asp³]MC-LR, MC-LR	[56]
66A	L. Sääskjärvi, Finland	1986	EU151874 EU151867	[Dha⁷]MC-HtyR, [D-Asp³, Dha⁷]MC-HtyR, [Dha⁷]MC-HphR, [Dha⁷]MC-LR, [L-Ser⁷]MC-HtyR	[56]
18B	L. Vaaranlampi, Finland	1986	EU151873 EU151866	[D-Asp³ Dha⁷]MC-RR, [Dha⁷]MC-RR	[56]
Microcystis
N-C 31	Little Rideau Lake, Canada	1954	EU009866 B EF115396	MC-LR	[15]
N-C 57	L. Frøylandsvatnet, Norway	1978	EU009867 C EF115397	[Asp³, Dha⁷]MC-RR, [Dha⁷]MC-RR	[15]
N-C 118/2	L. Gjersjøen, Norway	1983	EU009868 B EF115398	[Asp³]MC-LR, MC-LR	[15]
N-C 140	Bendig's Pond, Canada	1975	EU009869 B EU009881	MC-LR, MC-desmethyl-LR	This work
N-C 143	L. Akersvatnet, Norway	1984	EU009870 C EF115399	None	[15]
N-C 160/2	L. Akersvatnet, Norway	1985	EU009871 C EU009882	None	This work
N-C 161/1	L. Mosvatnet, Norway	1985	EU009872 B EF115400	MC-YR, MC-LR	[15]
N-C 169/7	L. Arresø, Denmark	1985	EU009873 C EF115401	MC-RR, MC-LR	[15]
N-C 171/10	L. Arresø, Denmark	1985	EU009874 C EU009883	MC-LR, MC-YR, MC-RR	This work
N-C 228/1	L. Akersvatnet, Norway	1985	EU009875 C EF115402	[Dha⁷]MC-RR, [Dha⁷]MC-LR	[15]
N-C 264	L. Frøylandsvatnet, Norway	1990	EU009876 C EF115403	[Dha⁷]MC-RR	[15]
N-C 324/1	L. Tøråssjøen, Norway	1993	EU009877 C EF115404	[Asp³, Dha⁷]MC-RR, [Dha⁷]MC-RR, [Dha⁷]MC-LR, MC-LR	[15]
N-C 357	River Zala, Hungary	1996	EU009878 C EU009884	MC-RR, MC-LR, MC-YR, MC-desmethyl-LR	This work
N-C 496	Queen Elizabeth Channel, Uganda	2004	EU009879 C EU009885	MC-YR, MC-desmethyl-YR	This work
AB2002/24	Pilsner Pond, Kenya	2002	EU009880 B EU009886	MC-LR, desmethyl-MC-YR, MC-YR	[57]
UV027	Germany	ND	AF458094 C AF458094	MC-RR	[9]
PCC 7806	Braakman Reservoir, The Netherlands	1972	AF183408 B AF183408	MC-LR, [Asp³]MC-LR	[10]
K-139	Lake Kasumigaura, Japan	1985	AB019578 B AB019578	[Dha⁷]MC-LR, [Asp³, Dha⁷]MC-LR	[58]
NIES 102	Lake Kasumigaura, Japan	1982	AB092807 C	MC-LR, MC-RR, MC-YR	[58]
Planktothrix
3	L. Mondsee, Austria	2001	AJ749276 AJ749285	[Asp³, Mdha⁷]MC-RR	[14]
64	L. Wörthersee, Austria	2001	AJ749277 AJ749286	[Asp³, Mdha⁷]MC-RR	[14]
111	L. Mondsee, Austria	2001	AJ749282 AJ749291	[Asp³, Mdha⁷]MC-RR	[14]
31/1	L. Wannsee, Germany	2001	AJ749267 AJ749294	[Asp³, Mdha⁷]MC-RR, [Asp³]MC-HtyR, [Asp³]MC-LR	[14]
32	L. Wannsee, Germany	2001	AJ749268 AJ749295	[Asp³, Mdha⁷]MC-RR, [Asp³]MC-LR	[14]
39	L. Wannsee, Germany	2001	AJ749269 AJ749296	[Asp³, Mdha⁷]MC-RR, [Asp³]MC-LR	[14]
79	L. Arresø, Denmark	2001	AJ749270 AJ749297	[Asp³, Mdha⁷]MC-RR, [Asp³]MC-LR	[14]
SAG 6.89	L. Plußsee, Plön, Germany	1969	AJ749271 AJ749298	[Asp³, Mdha⁷]MC-RR, [Asp³]MC-LR	[14]
N-C 126/8	L. Langsjön Finland	1984	AJ441056 AJ441056	[Asp³, Mdha⁷]MC-RR, [Asp³]MC-LR	[6]
80	L. Schwarzensee, Austria	2001	AJ749278 AJ749287	MC-HtyR	[11]
82	L. Ammersee, Germany	2001	AJ749279 AJ749288	[Asp³, Dhb⁷]MC-RR, [Asp³]MC-HtyR, [Asp³]MC-LR	[14]
108	L. Irrsee, Austria	2001	AJ749281 AJ749290	[Asp³, Dhb⁷]MC-RR, [Asp³]MC-LR	[14]
PCC 7821	L. Gjersjøen, Norway	1971	AJ749283 AJ749292	[Asp³, Dhb⁷]MC-RR, [Asp³]MC-LR	[14]
CCAP 1459/30	L. Plöner See, Germany	ND	AJ749284 AJ749293	[Asp³, Dhb⁷]MC-RR, [Asp³]MC-LR	[14]
CCAP 1459/11A	L. Windermere, UK	1975	AJ749272 AJ749299	[Asp³, Dhb⁷]MC-RR	[14]
CCAP 1459/21	Esthwaite Water, UK	1985	AJ749274 AJ749301	[Asp³, Dhb⁷]MC-RR	[14]
CCAP 1460/5	L. Kasumigaura, Japan	1983	AJ749275 AJ749302	[Asp³]MC-HtyR, [Asp³]MC-LR	[14]
CCAP 1459/16	Blelham Tarn, UK	1979	AJ749273 AJ749300	[Asp³]MC-HtyR, [Asp³]MC-LR	[14]

^#N-C, NIVA-CYA, Norwegian Institute for Water Research Cyanobacterial Culture Collection, PCC, Pasteur Culture Collection, NIES, National Institute for Environmental Studies Microbial Culture Collection, Japan, CCAP Culture Collection of Algae and Protozoa (Windermere, UK).
^&GenBank accession numbers for the microcystin synthetase genes analyzed. For each strain, the upper acc. no. indicates mcyB sequence.
* For Microcystis strains, the type of McyB A domain, B-type or C-like is indicated by B and C, respectively.
ND – no data available

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