Table 8 Primers used.
Primer | Sequence | Annealing temp (C°) |
|---|---|---|
Microcystis-mcyB-F (PCR and sequencing) | 5'-CCCAAGAGCAACATCAGTTATTAGT-3' | 58 |
Microcystis-mcyB-R (PCR and sequencing) | 5'-TTCCTGTCTATCTTGCCATTGTTA-3' | 57 |
Microcystis-mcyB-F2 (Sequencing) | 5'-AACGACTCCTGAGAATTTAGCCTAT-3' | 60 |
Microcystis-mcyB-R2 (Sequencing) | 5'-GTCAATTCAGGTTGGTTGAGGT-3' | 60 |
Microcystis-mcyC-F (PCR and sequencing)* | 5'-CAAGAAAAAGGCGTAACTTCAGA-3' | 55 |
Microcystis-mcyC-R (PCR and sequencing)* | 5'-AAGGTATCTTCCCGCATAATC-3' | 55 |
Anabaena-mcyB-F (PCR and sequencing) | 5'-TGATTTGAAAAGAAAGACCCAAT-3' | 56 |
Anabaena-mcyB-R (PCR and sequencing) | 5'-ATACCCAAACAAGAGTTGCTCAT-3' | 59 |
Anabaena-mcyB-F2 (Sequencing) | 5'-ACTTATCCGCTTATCGCAGGT-3' | 56 |
Anabaena-mcyB-R2 (Sequencing) | 5'-CCCAATATGTAATTCTCCAGCA-3' | 56 |
Anabaena-mcyC-F (PCR and sequencing) | 5'-CTCAATTCTGCTACTGTTGGTTTT-3' | 57 |
Anabaena-mcyC-R (PCR and sequencing) | 5'-CTTACCCACTAAAACCTCGAACT-3' | 54 |
Anabaena-mcyC-F2 (Sequencing) | 5'-AGGTAAGCCAAAGGGAGTGAT-3' | 57 |
Anabaena-mcyC-R2 (Sequencing) | 5'-CACCTCCAATATGTAATTCTCCA-3' | 57 |