Figure 3

Hybridization of PKD1 probes on gibbon and Old World monkey chromosomes. FISH of human PKD1 BAC clone RP11-304L19 (BAC) to metaphase chromosome 8 of the Mueller's gibbon (HMU) (a) and of human PKD1cDNA clone (cDNA) to metaphase chromosome 8 of the white-handed gibbon (HLA) (b). The identification of the distal short arm of chromosome 8 of both gibbon species as orthologous to human chromosome 16 is according to Jauch et al. [26] and Müller et al. [27]. Two-colour FISH of human BAC clone RP11-304L19 (BAC) and PKD1cDNA clone (cDNA) to metaphase chromosome 20 of the rhesus macaque (MMU) (c). FISH of human BAC clone RP11-304L19 (BAC) to prometaphase chromosome 20 of the proboscis monkey (NLA) (d). The identification of the chromosome 20 of the proboscis monkey as orthologous to human chromosome 16 is according to Bigoni et al. [31]. The colours of the inserted clone names correspond to those of the fluorescence signals (see Fig.1) on the DAPI-counterstained plates. Centromeres are marked by small bars.