Figure 4

Hybridization of PKD1 probes on New World monkey and lemur chromosomes. Two-colour FISH of human BAC clone RP11-304L19 (BAC) and PKD1-cDNA clone (cDNA) to metaphase chromosome 1 of the black-handed spider monkey (AGE) (a). The identification of the distal long arm of chromosome 1 of the spider monkey as orthologous to human chromosome 16 is according to Morescalchi et al. [32]. FISH of human BAC clone RP11-304L19 (BAC) to metaphase chromosome 24 of the brown lemur (EFU) (b), to metaphase chromosome 2 of the ring-tailed lemur (LCA) (c) and to metaphase chromosome 6 of the domestic dog (CFA) (d). The identification of the chromosome 24 of the brown lemur as orthologous to human chromosome 16 is according to Müller et al. [33], of the short arm of chromosome 2 of the ring-tailed lemur as orthologous to human 16p is according to Cardone et al.[34], and of chromosome 6 of the dog as orthologous to human 16p is according to Yang et al. [35]. The colours of the inserted clone names correspond to those of the fluorescence signals (see Fig.1) on the DAPI-counterstained plates. Centromeres are marked by small bars.