Figure 3

Amino-acid sequence comparison of acyl-CoA desaturases belonging to the Δ11-lepidopteran desaturase subfamily. The origin is indicated in the sequence according to: Cpa, Choristoneura parallela [25]; Cro, Choristoneura rosaceanea [20]; Ave, Argyrotaenia velutinana [23]; Epo, Epiphyas postvittana [24]; Onu, Ostrinia nubilalis [21]; Lca, Lampronia capitella (this study); Hze, Helicoverpa zea [18]; Sli, Spodoptera littoralis [28, 29]; Tni, Trichoplusia ni [17]; Tpi, Thaumetopoea pityocampa [30]; Bmo, Bombyx mori [27]; Mse, Manduca sexta [31] and Poc, Planotortrix octo [19]. The sequence name also refers to the biochemical activity as well as the GenBank accession number (Lca-KPVQ is referred to as LcaZ11-EU152335). Black and grey backgrounds indicate aa identities and conservative substitutions, respectively. Boxed regions indicate the three conserved HIS domains of desaturases, the four protein transmembrane domains (TM1 to TM4) and the Δ11-desaturase signature motif (SM), respectively [15]. The proposed ER retention signal [61] is underlined and marked in bold face (aa positions 356–359).