Table 1 Oligonucleotide primers used for desaturase gene characterization and functional expression

PR3

5'-GGYATYACVGCHGGNGCWCA-3'

PR4

5'-TGRTARTTRTGGAABSCYTCNCC-3'

Gene specific ^b

Primer sequence

Lc1–5a

5'-CAGGCCAGCGGCATCAGCAATAAGTA-3'

Lc1–5b

5'-CGAGGATTAATCTCAGCGGCCACT-3'

Lc1–3a

5'-TACTTATTGCTGATGCCGCTGGCCTGC-3'

Lc1–3b

5'-TCCTGGAAAAACGCGCTCTTCGTAGCTGCAATG-3'

Lc2–5a

5'-TCCTTTGGCCTTGATTTCGGGGTGTTTC-3'

Lc2–5b

5'-CCATGAGCCAACCCATGTGGGAGAAGAA-3'

Lc2–3a

5'-GGTGAAGAAACACCCCGAAATCAAGGCC-3'

Lc3–5a

5'-TCGCTCATGTCAATGCTGGCTCCCTTCT-3'

Lc3–5b

5'-ACGTTTGGCGTTGTGAGGATCAGCGTC-3'

Lc3–3a

5'-TTGAGGCGGACCCCATCGTCATGTTT-3'

Lc3–3b

5'-TGGTACGTGGCTACAATCCTGCGGTTCA-3'

Lc4–5a

5'-GTCAACAGCCAACCGACATGCGAA-3'

Lc4–5b

5'-GAGTCGGGATAACGAAGCAAACCA-3'

Lc4–3a

5'-TACGCAATTCCCTTCGTCGGATTG-3'

Lc4–3b

5'-CTTATGCCGATGTACTTCTGGAACG-3'

Full-length amplification ^b

Primer sequence

Lc1-fls

5'-GATTCATAGATTCGTGTTCGGTGA-3'

Lc1-flas

5'-CAGGGACCTCGAAGTGACCTTT-3'

Lc2-fls

5'-GCAGTGATTGGTGTCGTGCGGA-3'

Lc2-flas

5'-AACAATAAAATATTTATTTACATTAATTTGG-3'

Lc3-fls

5'-TAAAGCAAGTTACTGTCGCCG-3'

Lc3-flas

5'-GAAATACGTTTATTATTGGAATAAATAA-3'

Lc4-fls

5'-GAGTCACAAGTTGTTCAGTCGTAA-3'

Lc4-flas

5'-TCCATAAGATGGGTACGTATATTGT-3'

Expression YEpOLEX

Primer sequence ^c

Lc1ORFs

5'-gctctagaATGCCGCCGAACGTGACAG-3'

Lc1ORFas

5'-cgagctcgCTAATCATCTTTACGGTTAATG-3'

Lc2ORFs

5'-gctctagaATGCCGCCCGAGGGTCTAAT-3'

Lc2ORFas

5'-cgagctcgTCAAAAAGATTTCTCCGGAT-3'

Lc3ORFs

5'-gctctagaATGGCTCCAAACATCCTGGG-3'

Lc3ORFas

5'-cgagctcgTCACTGTTCTTTAGGATGTGC-3'

Expression pYEX

Primer sequence ^c

Lc4ORFs

5'-ggatccATAATGGCGCCATATCCTGAAG-3'

Lc4ORFas

5'-gaattcCGATTTCATATTAGTTCATTTTAATC-3'