The evolution of TEP1, an exceptionally polymorphic immunity gene in Anopheles gambiae

Table 3 McDonald and Kreitman tests of whether natural selection has driven the divergence of the TEP1s and TEP1r protein sequences.

Region analysed	n ^a	Codons^b	Ds	Ps	Dn	Pn	NI^c	p ^d
Whole CDS	29	1287	98	87	76	36	0.53	0.015
5' end (low divergence)	29	417	1	29	2	20	0.345	>0.5
3' end (high Divergence)	29	870	97	58	74	16	0.362	0.001
TED	85	272	30	23	42	12	0.373	0.024
remainder	29	598	65	43	30	10	0.500	0.123

Sequences of TEP1s and TEP1r were pooled across all populations and both species (An. gambiae and An. Arabiensis). Tests were conducted separately for regions that show high or low divergence between alleles, as well as separately for the TED region. The test is accomplished by comparing divergence and polymorphism at synonymous sites (Ps and Ds, respectively) to divergence and polymorphism at nonsynonymous sites (Dn and Pn, respectively)
a – Total number of haplotypes
b – Analysed codons
c – Neutrality Index
d – p-value 2-tailed fishers exact test

ISSN: 2730-7182