Figure 1

Bayesian phylogenetic tree of globins from C. elegans and from 30 other nematode, predominantly parasitic, species. Orthologs from parasitic species in clade II are marked in red. Clade I globins from parasitic nematodes that have been known for long because of their high abundance are marked in blue. The numbers at the nodes represent Bayesian posterior probabilities. All accession numbers from NEMBASE start with the initials of the species followed by P: Ancylostoma caninum (AC), Ancylostoma ceylanicum (AY), Ascaris lumbricoides (AL), Ascaris suum (AS), Dirofilaria immitis (DI), Globodera rostochiensis (GR), Haemonchus contortus (HC), Heterodera glycines (HG), Meloidogyne chitwoodi (MC), Meloidogyne hapla (MH), Meloidogyne incognita (MI), Meloidogyne javanica (MJ), Necator americanus (NA), Nippostrongylus brasiliensis (NB), Ostertagia ostertagi (OO), Onchocerca volvulus (OV), Parastrongyloides trichosuri (PT), Strongyloides stercoralis (SS), Toxocara canis (TC), Teladorsagia circumcincta (TD), Trichuris muris (TM), Trichinella spiralis (TS), Trichuris vulpis (TV), Xiphinema index (XI), Zeldia punctata (ZP). Plant, trematode (Platyhelminthes) and sea anemone (Cnidaria) globins were included as outgroup globins. The following plant globins (green) were included:Gossypium hirsutum (AAX86687), Malus domestica (AAP57676), Glycine max (AAA97887), Alnus firma (BAE75956), Citrus unshiu (AAK07675). The trematode globins (brown) were: Schistosoma japonicum (AAP06216), Paramphistomum epiclitum (AAG48877), Paragonimus westermani (AAX11352 and AAX11353), Clonorchis sinensis (AAM18464), Isoparorchis hypselobagri (P80722). Nematostella vectensis globins (purple): Nemvec141000032, Nemvec3000224, Nemvec50000067, Nemvec7000121 and Nemvec76000030.