Figure 3

WOX13 OG gene expression in O. tauri, P. patens and A. thaliana. (A) RT-PCR analysis of WOX gene expression in Ostreococcus tauri. 12 h light/dark synchronized or continuous light (cont) cell cultures of 10-day-old were used. Gene name (left side), number of PCR cycles done (right side), time of sampling (top side), light (blank bar) and dark (black bar) periods. The genes OtRH4 and OtRH21 were used as constitutive expression controls. RT were done using oligodT primers except in (*) where random primers were used (B) RT-PCR analysis of WOX gene expression during Physcomitrella patens development: (1) 4-day-old, (2) 10-day-old, (3) 14-day-old, (4) genomic DNA control. (C) RT-PCR analysis of the expression of WOX13 OG genes carried out on 7-day old seedlings of different Arabidopsis thaliana lines: (1) Wild type No ecotype, (2) the pst13645 AtWOX14 mutant line, (3) Wild type WS ecotype, (4) the 193G10 AtWOX13 mutant line, (5) Genomic DNA control.