Figure 3From: Evolutionary conservation of dual Sec translocases in the cyanelles of Cyanophora paradoxaA. Fractions obtained from cyanelle lysate by sucrose density gradient floatation centrifugation. B. Binding of 35S-labeled pre-FNR from C. paradoxa to isolated cyanelles under non-import conditions, lysis and fractionation. Aliquots of the fractions (40% of 1, 5% of 2, 5% of 3, 30% of 4 and 50% of 5) were precipitated with TCA and run on a SDS gel. Radioactivity in the pellet (fraction 5) is likely bound to yet intact cyanelles. The band at 35 kDa represents a degradation product [42] rather than processed mature protein.Back to article page