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Figure 4 | BMC Evolutionary Biology

Figure 4

From: Evolutionary conservation of dual Sec translocases in the cyanelles of Cyanophora paradoxa

Figure 4

Separation of protein complexes from the OEM and periplasm of cyanelles by BN-PAGE applied under gentle (hypertonic) conditions where no noticeable lysis of IEM and liberation of stromal proteins occurred. A. Intact, import-competent cyanelles (500 μg chlorophyll) were treated with (1) 1.67% digitonin, (2) 1.67% digitonin and 0.5% dodecyl maltoside and (3) 1.67% digitonin and 0.03% Triton X-100. B. Western blot of lane 1 (αToc75-III).

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