Group I introns and associated homing endonuclease genes reveals a clinal structure for Porphyra spiralis var. amplifolia (Bangiales, Rhodophyta) along the Eastern coast of South America

Table 3 Seventeen haplotypes (H1 to H17) distributed over 11 Porphyra spiralis var. amplifolia (PSA) populations (Table 1).

Haplotype/Intron size	PSA Populations	ORF	His-Cys
H1 - 1056 bp	PSA-A	137 aa	Yes, f/s
H2 - 1058 bp	PSA-I	138 aa	Yes
H3 - 1054 bp	PSA-R^1,2	137 aa	Yes, f/s
H4 - 1055 bp	PSA-L	137 aa	Yes
H5 - 1055 bp	PSA-I, -L, -T	137 aa	Yes
H6 - 909 bp	PSA-D¹	87 aa	Yes, f/s
H7 - 909 bp	PSA-S, -T	88 aa	Yes, f/s
H8 - 791 bp	PSA-C	49 aa	No
H9 - 792 bp	PSA-G	50 aa	No
H10 - 792 bp	PSA-G, -S, -T	50 aa	No
H11 - 792 bp	PSA-T	50 aa	No
H12 - 792 bp	PSA-S	50 aa	No
H13 - 791 bp	PSA-S	50 aa	No
H14 - 744 bp	PSA-B¹	45 aa	No
H15 - 616 bp	PSA-V	8 aa	No
H16 - 616 bp	PSA-V	8 aa	No
H17 - 616 bp	PSA-V	8 aa	No

Intron sizes in base pairs (bp), homing endonuclease open reading frames (ORF) sizes in amino acids (aa) and if the His-Cys box motif is present are indicated; f/s indicates if frame shifts corrections are necessary for establishing the correct His-Cys box reading frame. Data obtained from: ¹ Oliveira and Ragan [2], ² Haugen et al [4].

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