Spatial expression pattern of AmSoxF throughout development. An asterisk indicates the blastopore. (C-H) Oral pore is oriented to the left and aboral side to the right. (A) Unfertilized egg. (B) late donut stage (C-H) weak endodermal expression appears at the pre-pear stage, strengthening throughout the late pear and planula stages. (I-J) Endodermal expression continues post-settlement.