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Table 1 Primers used in this study.

From: Transfer of rice mitochondrial ribosomal protein L6 gene to the nucleus: acquisition of the 5'-untranslated region via a transposable element

Primer

Sequence

P1

5'-CCTCTTCTCTTGTTGccATGGAG-3'

P2

5'-CAGTACAGCAAATTAccaTGGGTTTG-3'

P3

5'-TTTCCCTGTCTTATTGCcATGGAA-3'

P4

5'-CCAGTACAGCATATTAccaTGGGTTTA-3'

P5

5'-CTGCTTCAAACtgtACaTAATTTGC-3'

P6

5'-GAAAGTAAAATgcGGccGCTCATCC-3'

P7

5'-TTGTTTCTGAAACTTGGTTACAG-3'

P8

5'-TTACTTCTTTTGCTTCTTCCCTGGCTT-3'

P9

5'-AATGG(A/C)AC(C/T)G(A/G)(A/T)ATGGTCAAG-3'

P10

5'-TTAGAAGCA(C/T)TTC(A/C)TGTG(C/G)AC-3'

  1. Underline: restriction sites for BsrGI, NcoI and NotI.
  2. Lowercase letters: deviations from the original OsRpl6-1 sequence to enable introduction of restriction sites.
  3. The locations of primers P1-P8 are shown in Figure 1A.
  4. Primers P9 and P10 were designed from rice Actin sequences [39] as described previously [40] with slight modifications.