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Table 1 Primers and PCR conditions.

From: Evolutionary history of the fish genus Astyanax Baird & Girard (1854) (Actinopterygii, Characidae) in Mesoamerica reveals multiple morphological homoplasies

Gene

Primers

Sequence (5'-3')

Tm (°C)

Size (pb)

Reference

RAG1

RAG1 (a, s)

AGCTGTAGTCAGTAYCACAARATG

   
 

RAG5 (s)

TRGAGTCACACAGACTGCAGA

58*

1512

[67].

 

RAG9 (a, s)

GTGTAGAGCCAGTGRTGYTT

   

Cytochrome oxidase I (COI)

FISHF1 (a, s)

TCAACCAACCACAAAGACATTGGCAC

   
 

FISHR1 (a)

TAGACTTCTGGGTGGCCAAAGAATCA

54

655

[83]

Cytochrome b (Cytb)

Glu- F (a, s)

GAAGAACCACCGTTGTTATTCAA

   
 

Thr- R (a, s)

ACCTCCRATCTYCGGATTACA

48

 

[84]

 

CbCHR (a, s)

TTARTCCGGCTGGGWTNTTTG

48

1140

This study

16 S

16SAR (a, s)

CGCCTGTTTATCAAAAACAT

   
 

16SBR (a)

CCGGTCTGAACTCAGATCACGT

46

552

[85].

  1. Amplification (a) and sequencing (s) primers used for the RAG1, 16S, COI, and Cytb genes. *Touchdown PCR was performed for the first 10 cycles: from 58°C to 53°C (-0.5°C each cycle), followed by 25 cycles at 53°C.