|  | Estimated ages | ||
---|---|---|---|---|
Node | Calibration point | Strict Molecular Clock | Relaxed Molecular Clock NPRS with r8s Mya | |
 |  | 0.8%/Mya | 1.05%/Mya | 95% of confidence |
1 | Merida-Perija Mountains 8–12 Mya | 24.6 ML (18 – 23) | 32.3 ML (23.7–42.8) | 15 |
 | Colossoma macropomum 15 Mya | 11 p (9.8 – 12.4) | 14.6 p (12.8–16.3) |  |
2 | Mesoamerica Colonization | 7.7 ML (6.36–9.32) | 10.05 ML (8.35–12.23) | 7.9 SD 0.165 (7.8–8.1) |
 |  | 5.5 p (4.9–6.4) | 7.3 p (6.4–8.5) |  |
3 | Motagua-Polochic fault | 4.6 ML (2.9–7.9) | 6 ML (3.8–10) | 7.1* SD 0.34 (6.0–7.8) |
 |  | 3.7 p (2.5–5.7) | 4.9 p (3.3–7.5) |  |
4 | Radiation in North Mesoamerica Lineages | 3.8 ML (2–6.2) | 4.83 ML (2.7–8.2) | 6.7* SD 0.37 (5.7–7.5) |
 |  | 3.1 p (1.9–4.8) | 4.1 p (2.5–6.3) |  |
5 | Central America Radiation | 3.7 ML (2–6.2) | 5 ML(0.2–7.42) | 6.9* SD 0.39 (5.9–7.7) |
 |  | 3.1 p (1.9–4.8) | 4.2 p (0.2–5.5) |  |
6 | TMVB 3–6 Mya | 3.6 ML (2.3–5.4) | 4.7 ML (3.1–7) | 5.25* SD 0.37 (4.4–6.2) |
 |  | 3.07 p (2.2–4.3) | 4.03 p (2.8–5.7) |  |