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Table 2 Sequence data and polymorphism parameters of the four amplified immunity genes in the species of the An. gambiae complex. Length of sequences, exons and number of exons refer to the fragments sequenced in this study. Alleles were inferred based on the coding regions of the sequences.

From: The molecular evolution of four anti-malarial immune genes in the Anopheles gambiae species complex

  Number of individuals Length of sequences obtained (bp) Number of exons/length of each exon sequence (bp) analysed Number of sequences obtained per species (number of alleles per species) Polymorphic sites Nucleotide diversity (Pi)
      Syn. Non-Syn. Syn. Non-Syn.
Gambicin
An. arabiensis 6 646–677 3/75, 90, 81 10 (8) 5 5 0.002 0.007
An. bwambae 6 667–675 3/75, 90, 81 11 (7) 4 5 0.020 0.006
An. gambiae 5 666–672 3/75, 90, 81 9 (8) 4 4 0.024 0.006
An. melas 6 589–672 3/75, 90, 81 11 (4) 0 5 0.000 0.004
An. merus 5 664–682 3/75, 90, 81 8 (4) 2 1 0.008 0.001
An. quadriannulatus 5 663–682 3/75, 90, 81 10 (6) 5 4 0.019 0.004
Total 33    59 (37)     
NOS
An. arabiensis 5 997–1006 3/201, 432, 138 5 (5) 19 2 0.050 0.001
An. bwambae 7 997–1008 3/201, 432, 138 11 (8) 10 7 0.021 0.003
An. gambiae 6 1006–1007 3/201, 432, 138 7 (7) 16 6 0.029 0.003
An. melas 5 1004–1007 3/201, 432, 138 8 (5) 3 4 0.007 0.002
An. merus 6 993–1001 3/201, 432, 138 10 (8) 15 4 0.028 0.001
An. quadriannulatus 5 1002–1006 3/201, 432, 138 5 (5) 7 2 0.019 0.001
Total 34    46 (38)     
Rel2
An. arabiensis 10 780–785 2/372, 339 20 (18) 15 10 0.031 0.004
An. bwambae 6 712–778 2/372, 339 6 (1) 0 1 0.000 0.000
An. gambiae 14 732–794 2/372, 339 16 (11) 19 6 0.020 0.004
An. melas 8 761–800 2/372, 339 10 (5) 3 2 0.008 0.001
An. merus 7 665–796 2/372, 339 11 (6) 2 4 0.003 0.002
An. quadriannulatus 8 762–803 2/372, 339 11 (8) 9 4 0.013 0.002
Total 53    74 (49)     
FBN9
An. arabiensis 7 789–807 1/807 12 (12) 40 7 0.065 0.003
An. bwambae 7 787–807 1/807 8 (3) 18 2 0.040 0.001
An. gambiae 7 764–807 1/807 12 (12) 41 1 0.079 0.000
An. melas 7 776–791 1/791 7 (7) 9 0 0.021 0.000
An. merus 7 788–807 1/807 11 (10) 10 4 0.016 0.002
An. quadriannulatus 7 780–807 1/807 10 (10) 18 6 0.034 0.002
Total 42    60 (54)