Table 2 Sequence data and polymorphism parameters of the four amplified immunity genes in the species of the An. gambiae complex. Length of sequences, exons and number of exons refer to the fragments sequenced in this study. Alleles were inferred based on the coding regions of the sequences.
From: The molecular evolution of four anti-malarial immune genes in the Anopheles gambiae species complex
| Â | Number of individuals | Length of sequences obtained (bp) | Number of exons/length of each exon sequence (bp) analysed | Number of sequences obtained per species (number of alleles per species) | Polymorphic sites | Nucleotide diversity (Pi) | ||
|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Â | Syn. | Non-Syn. | Syn. | Non-Syn. |
Gambicin | ||||||||
An. arabiensis | 6 | 646–677 | 3/75, 90, 81 | 10 (8) | 5 | 5 | 0.002 | 0.007 |
An. bwambae | 6 | 667–675 | 3/75, 90, 81 | 11 (7) | 4 | 5 | 0.020 | 0.006 |
An. gambiae | 5 | 666–672 | 3/75, 90, 81 | 9 (8) | 4 | 4 | 0.024 | 0.006 |
An. melas | 6 | 589–672 | 3/75, 90, 81 | 11 (4) | 0 | 5 | 0.000 | 0.004 |
An. merus | 5 | 664–682 | 3/75, 90, 81 | 8 (4) | 2 | 1 | 0.008 | 0.001 |
An. quadriannulatus | 5 | 663–682 | 3/75, 90, 81 | 10 (6) | 5 | 4 | 0.019 | 0.004 |
Total | 33 | Â | Â | 59 (37) | Â | Â | Â | Â |
NOS | ||||||||
An. arabiensis | 5 | 997–1006 | 3/201, 432, 138 | 5 (5) | 19 | 2 | 0.050 | 0.001 |
An. bwambae | 7 | 997–1008 | 3/201, 432, 138 | 11 (8) | 10 | 7 | 0.021 | 0.003 |
An. gambiae | 6 | 1006–1007 | 3/201, 432, 138 | 7 (7) | 16 | 6 | 0.029 | 0.003 |
An. melas | 5 | 1004–1007 | 3/201, 432, 138 | 8 (5) | 3 | 4 | 0.007 | 0.002 |
An. merus | 6 | 993–1001 | 3/201, 432, 138 | 10 (8) | 15 | 4 | 0.028 | 0.001 |
An. quadriannulatus | 5 | 1002–1006 | 3/201, 432, 138 | 5 (5) | 7 | 2 | 0.019 | 0.001 |
Total | 34 | Â | Â | 46 (38) | Â | Â | Â | Â |
Rel2 | ||||||||
An. arabiensis | 10 | 780–785 | 2/372, 339 | 20 (18) | 15 | 10 | 0.031 | 0.004 |
An. bwambae | 6 | 712–778 | 2/372, 339 | 6 (1) | 0 | 1 | 0.000 | 0.000 |
An. gambiae | 14 | 732–794 | 2/372, 339 | 16 (11) | 19 | 6 | 0.020 | 0.004 |
An. melas | 8 | 761–800 | 2/372, 339 | 10 (5) | 3 | 2 | 0.008 | 0.001 |
An. merus | 7 | 665–796 | 2/372, 339 | 11 (6) | 2 | 4 | 0.003 | 0.002 |
An. quadriannulatus | 8 | 762–803 | 2/372, 339 | 11 (8) | 9 | 4 | 0.013 | 0.002 |
Total | 53 | Â | Â | 74 (49) | Â | Â | Â | Â |
FBN9 | ||||||||
An. arabiensis | 7 | 789–807 | 1/807 | 12 (12) | 40 | 7 | 0.065 | 0.003 |
An. bwambae | 7 | 787–807 | 1/807 | 8 (3) | 18 | 2 | 0.040 | 0.001 |
An. gambiae | 7 | 764–807 | 1/807 | 12 (12) | 41 | 1 | 0.079 | 0.000 |
An. melas | 7 | 776–791 | 1/791 | 7 (7) | 9 | 0 | 0.021 | 0.000 |
An. merus | 7 | 788–807 | 1/807 | 11 (10) | 10 | 4 | 0.016 | 0.002 |
An. quadriannulatus | 7 | 780–807 | 1/807 | 10 (10) | 18 | 6 | 0.034 | 0.002 |
Total | 42 | Â | Â | 60 (54) | Â | Â | Â | Â |