Evolution of the AZFc amplicons in the primate lineage. (A) The prototype architecture of the AZFc region on the human Y chromosome. The amplicons are color-coded according to . The locations of the hybridization probes within the various amplicons are indicated above with vertical arrows, and the DAZ and the RBMY genes are indicated underneath. Autosomal homologous regions are shown at the bottom as colored horizontal lines with the chromosome numbers indicated underneath. Broken lines represent discontinuous homology. 1* indicates that in addition to chromosome 1, several other autosomes also share homology with the region. (B) – (G) Southern analyses of the presence of sequences homologous to the various amplicons on primate Y chromosomes. Human (Hsa), bonobo (Ppa), chimpanzee (Ptr), gorilla (Ggo), orangutan (Ppy), rhesus monkey (Mmu), pig-tail macaque (Mne), spider monkey (Age), marmoset (Cge), capuchin (Cca), galago (Gal) and lemur (Lca) genomic DNAs were digested with either HindIII or NsiI and hybridized with probes from the various amplicons as indicated. Yellow-amplicon-15 and yellow-amplicon-1 contain sequences homologous to chromosome 15 and chromosome 1, respectively. The Y chromosome fragments are marked with red asterisks. F: female, M: male.