Unveiling an ancient biological invasion: molecular analysis of an old European alien, the crested porcupine (Hystrix cristata)

Table 1 Sequences and references of primers used in this study.

Gene	Primer	Sequence (5'-3')	Ta °C	Ref.
CR	Hy-For1	CACCATCAGCACCCAAAG	56	[34]
	Hy-For2	AAACCAGCAACCCGACAG	56	[34]
	Hy-For3	AATTGTAGCTGGACTTATAAATC	56	[34]
	Hy-For4	CCCCCGTAAATTTAATAGCTT	56	[34]
	Hy-Rev1	AAGCATTTTCAGTGCTTTRC	56	[34]
	Hy-Rev2	GGGGGTTTGTCAATAGATTG	56	[34]
	Hy-Rev3	GTCCTTCAAGCATTAAAAGAAATC	56	[34]
	Hy-Rev4	AGAAGAGGGATCCCTGTC	56	[34]
	Hy-For5	AATGCAAGACCCCATAAGAC	56	[34]
	Hy-Rev5	GAGTGGGCGATTTTAAGTGT	56	[34]
12S	12S-L82	CATAGACACAGAGGTTTGGTCC	54	[35]
	12S-H293	GCACGAGATTTACCAAC	54	[35]
	12S-H618	TATCGATTATAGAACAGGCTCC	54	[35]
Cyt b	Cyt01F	GAACTAATGACAAACATCCGAAAA	54	This study
	Cyt05F	AACACGATTCTTTGCTTTCCA	54	This study
	Cyt06R	TGGACTAGTACAAGGGCTGTGA	54	This study
	Cyt02R	TTCTGGTTTAATATGGGGAGGA	54	This study

CR: Control Region; 12S: rRNA 12S; Cyt b: Cytochrome b; Ta: PCR annealing temperature.

ISSN: 2730-7182