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Figure 3 | BMC Evolutionary Biology

Figure 3

From: Evidence for increased olfactory receptor gene repertoire size in two nocturnal bird species with well-developed olfactory ability

Figure 3

Southern hybridization of restriction enzyme digested palaeognath genomic DNAs. Genomic DNAs isolated from five palaeognath taxa (ostrich, emu, brown kiwi, greater rhea, elegant-crested tinamou) were digested with four different restriction enzymes and used for Southern hybridization with four DIG-labeled probes generated from elegant-crested tinamou partial OR coding sequences: (A) probe Tin-A [GenBank: EU599489], (B) probe Tin-B [GenBank: EU599490], (C) probe Tin-C [GenBank: EU599491] and (D) probe Tin-D [GenBank: EU599492]. 37°C hybridization conditions were used for the results shown. Abbreviations: T, TaqI; P, PstI; E, EcoRI; H, HindIII; DIG, digoxigenin; Tin, elegant-crested tinamou. Approximate positions of the size standards (kb) are indicated. Note that each figure was generated by splicing together images from two separate blots (see Methods for details).

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BMC Ecology and Evolution

ISSN: 2730-7182

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