Figure 6

Effects of RNA interference of genes coding for asymmetric and symmetric classes of epiplasmins on P. tetraurelia. A-Control Cells: a) Low magnification phase contrast and immunofluorescence with mAb CTS32 counterstained with DAPI. b) Higher magnification showing the alignment of cortical units along the kinetids. The arrow heads show normal pattern of scale duplication. B-Cells submitted to Epi 2 RNAi: a) Cell shape alteration at division time (boomerang) and 24 h later (plasmodial form). b) Irregularities in the alignment and the shape of the scales, and abnormally oriented striction of scales leading to 'double kinetids' (circled with white line) C-Cells submitted to Epi 41 RNAi: a) Same cell shape alteration as with Epi 2 at division time (boomerang) and 24 h later (plasmodial form). b) Numerous granules of epiplasmic material surround crenulated scales.