Figure 4

An abridged multiple sequence alignment with putative functional site tallies. For clarity, only the consensus sequence along with representative Hmd enzyme and paralog₁ sequences from M. jannaschii are shown. The original multiple sequence alignment containing every sequence is available as Additional file 6. Arrows indicate positions where at least one top ten MFS scoring residue appears in the alignment. Such residues are considered putative functional sites. Arrows referring to putative functional residues from Hmd enzymes are shown above the alignment while those referring to putative functional residues from the Hmd paralogs are shown below the alignment. Numbers at the base of each arrow refer to the quantity of putative functional residues that appear in a single alignment position. Putative functional residues from models that had both the best C-score [27] and RAPDF score [30] are counted twice. Positions in which putative functional residues are found in at least 40% of either Hmd enzymes or Hmd paralogs are highlighted. In five such alignment positions, putative functional sites are found in at least 40% of Hmd enzymes and 40% of Hmd paralogs. Such residue positions are predicted to facilitate a function that is common between Hmd enzymes and paralogs.