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Table 1 Composition of experimental diets (g/100 g of dry diet)

From: Differential transcriptional modulation of duplicated fatty acid-binding protein genes by dietary fatty acids in zebrafish (Danio rerio): evidence for subfunctionalization or neofunctionalization of duplicated genes

Ingredient

HD*

LD*

LND*

LFD*

Vitamin free Casein1

33

33

33

33

Wheat Gluten2

10

10

10

10

Gelatin1

4

4

4

4

Flax oil3

2

2

8

2

Coconut oil3

2

2

2

-

Omega Mix4

6

-

-

-

Sunflower oil3

2

8

2

2

Corn starch (pre-gelatinized)5

29.1

29.1

29.1

37.1

Celufil1

8

8

8

8

Vitamin Mix6

1.3

1.3

1.3

1.3

Mineral Mix7

1

1

1

1

Betaine8

1.5

1.5

1.5

1.5

DL-Methionine1

0.2

0.2

0.2

0.2

Total

100

100

100

100

  1. * HD - highly unsaturated fatty acids rich diet; LD - linoleic acid rich diet; LND - linolenic acid rich diet; LFD - low fat diet; BHA-butyl hydroxy anisole.
  2. 1US Biochemical, Cleveland, OH, USA.
  3. 2Dover Mills Ltd. (Halifax, NS, Canada).
  4. 3Obtained from the local market.
  5. 4Jamieson omega-3 select capsules.
  6. 5National Starch and Chemical Co. (Bridgewater, NJ, USA).
  7. 6Vitamin added to supply the following (per kg diet): vitamin A, 8000 IU; vitamin D3, 4000 IU; vitamin E, 300 IU; vitamin K3, 40 mg; thiamine HCl, 50 mg; riboflavin, 70 mg; d-Ca pantothenate, 200 mg; biotin, 1.5 mg; folic acid, 20 mg; vitamin B12, 0.15 mg; niacin, 300 mg; pyridoxine HCl, 20 mg; ascorbic acid, 300 mg; inositol, 400 mg; choline chloride, 2000 mg; butylated hydroxy toluene, 15 mg; butylated hydroxy anisole, 15 mg.
  8. 7Mineral added to supply the following (per kg diet): manganous sulphate (32.5% Mn), 40 mg; ferrous sulphate (20.1% Fe), 30 mg; copper sulphate (25.4% Cu), 5 mg; zinc sulphate (22.7% Zn), 75 mg; sodium selenite (45.6% Se), 1 mg; cobalt chloride (24.8% Co), 2.5 mg; sodium fluoride (42.5% F), 4 mg.
  9. 8Betaine anhydrous (96% feed grade), Finnfeeds, Finland.