Isolate | PPE39 | PPE40 | Comment |
---|---|---|---|
M. bovis | Deleted downstream from position 1358. | 3 bp in-frame deletion removes aa 164 (A). | PPE39 part of RD5 region (Figure 3). |
M. bovis BCG | PPE39/40 gene fusion. 3 bp in-frame deletion seen in M. bovis PPE40 also present. | Â | Fused PPE39/40 (Figure 3). |
CPHL_A | + | IS6110 integration at position 47. | PPE40 function predicted to be abolished. |
K85 | sSNP G→ T position 1548 | + |  |
GM041182 | sSNP C→ T position 1563. | 33bp in-frame deletion of nucleotides 190 -- 222. Removes aa sequence AAAAAMVVAAA. | PPE40 function predicted to be altered. |
M. microti | Deleted downstream of position 325. | + | PPE39 is part of the RD5micregion (Figure 3). |
Oryx bacillus | Deleted | Deleted | PPE39 and PPE40 are parts of the RD5oryxregion (Figure 3). |
Dassie bacillus | Deleted | Gene present. | Deletion analysis suggests that PPE40 is intact but exact sequence is unknown. |
T17 | + | + | Â |
EAS054 | 3 bp (GCG) in-frame deletion removes alanine at aa position 27. | + | Â |
T92 | Deleted | Deleted from position 1592. | See Figure 3. |
94_M4241A | PPE39/40 gene fusion. | Â | Fused PPE39/40 (Figure 3). |
02_1987 | Deleted | IS6110 integration at position 47. 3' region of gene deleted. | Most of genes deleted as part of major genomic structural alterations. (Figure S23). |
T85 | G insertion position 830, A deletion position 942. Stop codon aa position 278. | + | PPE39 function predicted to be abolished or highly modified. |
KZN 4207 | + | + | Â |
KZN 1435 | + | 6 nsSNPs between positions 1094 and 1105. 2 aa changes: 367 T→ N and 368 G→ N. |  |
KZN 605 | + | + | Â |
F11 | IS6110 integration at position 47. | + | PPE39 function predicted to be abolished. |
Strain C | N.D. | sSNP T→ C position 969. | Unable to characterise PPE39 sequence due to the numerous 'N's'. Full length gene present. |
CDC1551 | 3 bp (GCG) in-frame deletion removes alanine at aa position 27. | + | Â |
Haarlem | IS6110 integration at position 47. | + | PPE39 function predicted to be abolished. |
H37Rv | IS6110 integration at position 20. Following IS PPE39 sequence commences at position 821. | + | PPE39 function predicted to be abolished. |
H37Ra | As for H37Rv. | + | PPE39 function predicted to be abolished. |