Figure 4

Gene-specific semiquantitative RT-PCR on RNA prepared from dissected Opithandra dinghushanensis flowers. A) sepal (S), dorsal petal+staminode (Dp+s), lateral/ventral petals (Lp/Vp), lateral stamens (Ls) and ventral staminode (Vs) were dissected from flower buds of middle-stage (less than 1 cm long). B) sepal (S), dorsal petal+staminode (Dp+s), lateral/ventral petals (Lp/Vp), lateral stamens (Ls) and ventral staminode (Vs) were dissected from flowers of late-stage (3-4 cm long). C) Relative level of OpdCYC1C mRNA expression in middle-stage (black) vs. late-stage (grey) compared with ACTIN. D) Relative level of OpdCYC2A mRNA expression in middle-stage (black) vs. late-stage (grey) compared with ACTIN. E) Relative level of Opdcyclin D3a mRNA expression in middle-stage (black) vs. late-stage (grey) compared with ACTIN. F) Relative level of Opdcyclin D3b mRNA expression in middle-stage (black) vs. late-stage (grey) compared with ACTIN. ACTIN was used for RT template control. The values (means ± SD) shown are determined from five independent experiments.