Species delimitation in lemurs: multiple genetic loci reveal low levels of species diversity in the genus Cheirogaleus

Table 5 Primers used in this study

Locus	Primer	Primer sequence 5' - 3'	Reference	°C
cytb	L-CYT	AAT GAT ATG AAA AAC CAT CGT TGT A	[64]	55
	2763	GG(AG) ATT TT(AG) TCG GAG TCT GAT G	this study
	2695	CCG ATT CTT CGC ATT CCA CTT	this study	55
	2510	GAC CAG (GT)GT ATT (AT)TT TAT ACT AC	C. Roos, pers. comm.
	2877	ACG TAA AC(CT) ACG GCT GAA	this study	52
	2879	CCT CAG ATT CAT TCT ACT A	this study
cox2	L7553	AAC CAT TTC ATA ACT TTG TCA A	[65]	48
	H8320*	CTC TTT AAT CTT TAA CTT AAA AG	[65]
adora3	adora3F	ACC CCC ATG TTT GGC TGG AA	[66]	52
	adora3R	GAT AGG GTT CAT CAT GGA GTT	[67]
fiba	Fiba-F	AAG CGC AAA GTC ATA GAA AAA G	[66]	56
	Fiba-R	CTA AAG CCC TAC TGC ATG ACC CT	[66]
vWF	vWF-10	GAG CTG GAT GTC CTG GCC ATC CAT GGC AAC	[68]	60
	vWF-8	GAG TGC CTT GTC ACT GGT CAT CCC ACT TCA A	[68]

The locus, primer name, primer sequence, reference and used annealing temperature in °C are given. The complete cytb locus was obtained by amplifying two overlapping fragments (primer pairs 1255, 2763 and 2695, 2510). The cytb fragments from museum material were amplified using primer pair 2877, 2879. *slightly modified from Adkins and Honeycutt(1994), fourth base not present in original primer.

ISSN: 2730-7182