Figure 4

Supporting phylogenetic reconstructions displaying the relationships of vertebrate Akirin1 and Akirin2 proteins and the single Akirin protein of four invertebrate deuterostomes. The corresponding amino acid sequence alignment is as in fig. 3. (a) Is an unrooted NJ tree produced using the JTT model and implementing a gamma distribution parameter as estimated by ML. (b) Is a NJ tree reconstructed from solely the unsaturated fraction of sites, using the program ASATURA [11] with the JTT model. (c) Is an unrooted ME tree produced using the JTT model and implementing a gamma distribution parameter as estimated by ML. (d) Is an unrooted MP tree constructed using the close-neighbour-interchange method. Each analysis supported the topologies returned by ML and Bayesian approaches (fig. 3). Branch support values represent a percentage of 1000 bootstrap replicates.