Allopatric speciation in ticks: genetic and reproductive divergence between geographic strains of Rhipicephalus (Boophilus) microplus

Table 5 Genotyping of microsatellite loci in geographic strains of R. microplus.

Tick strains^a	Microsatellite loci (repeated array)
	BmA12 (CA)₃₊₇(CG)₄		BmA06 (GT)₈₊₂		BmB12 (TA)₄(TG)₉		BmC03 (CA)₁₀₊₉		BmC07 (GT)₁₇		BmD12 (CA)₁₀₊₅
	Na	SR (bp)	Na	SR (bp)	Na	SR (bp)	Na	SR (bp)	Na	SR (bp)	Na	SR (bp)
MOZ × MOZ	1	94	ND	ND	3	[180–216]	5	[157–171]	4	[132–192]	2	[93; 95]
ARG × ARG	1	94	2	[99; 101]	5	[180–216]	2	[173; 175]	1	144	1	93
AUS × AUS	4	[94–200]	4	[96–102]	4	[191–199]	4	[146–158]	3	[144–180]	4	[88–105]
MOZ × ARG	1	94	2	[99; 101]	4	[180–216]	6	[165–175]	5	[132–192]	5	[93–109]
AUS × ARG	4	[94–200]	5	[96–101]	6	[186–214]	5	[146–175]	5	[132–150]	8	[88–112]
AUS × MOZ	3	[94–198]	3	[98–102]	4	[180–199]	6	[157–171]	8	[144–192]	4	[88–109]
India	3	[198–218]	2	[108; 204]	1	180	ND	ND	ND	ND	ND	ND
New Caledonia	1	200	1	108	4	[176–199]	ND	ND	2	[146; 150]	1	107

^aFor Argentinean (ARG), Australian (AUS) and Mozambican (MOZ) strains, egg masses from two separate crosses were used for DNA extraction and genotyping following published procedures (22). For homologous and heterologous crosses, female_{strain 1} × male_{strain 2} and female_{strain 2} × male_{strain 1} crosses were used. For India and New Caledonia strains, pooled tick larvae and adult ticks were used for analysis, respectively. ND, not determined; Na, number of alleles; SR, allele size range.

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