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Table 1 Microsatellite loci assayed from adult Gobiusculus flavescens.

From: Multiple mating and a low incidence of cuckoldry for nest-holding males in the two-spotted goby, Gobiusculus flavescens

Locus

Primer sequence (5'-3')

Repeat Motif

Temp (°C)

N

A

H O

H E

Excl. prob

2SG-08

F: TGATGGTTCTTCTTTCAATATGC

R: GCTGCTGGACACCTGAATTT

(GATA)13

58

33

18

0.848

0.908

0.811

2SG-17

F: GCTGCTGGACACCTGAATTT

R: CGATCGCCTTTCAGTTTGAC

(CTAT)11

56

33

15

0.788

0.763

0.592

2SG-21

F: TGTAGGTGCCTTCCCCATTA

R: GGACTCCTGCATCTCTGCAT

(GATA)10

58

33

16

0.758

0.842

0.685

2SG-55

F: CATACATGCGTGCTCAAAAA

R: TGTCGGTATTGAAACATCCAA

(NTAT)14 a

59

31

13

0.839

0.821

0.650

  1. aActual sequence = (CTAT)4GTAT(CTAT)2(GTATCTAT)2(CTAT)3
  2. Name of locus, primer sequence of the original cloned microsatellite, PCR reannealing temperature (°C), number of adults assayed (N), number of alleles per locus (A), observed (HO) and expected heterozygosity (HE) and exclusion probabilities (Excl. prob; given one parent known with certainty and the second parent unknown) are listed for each locus.
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BMC Ecology and Evolution

ISSN: 2730-7182

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