Multiple mating and a low incidence of cuckoldry for nest-holding males in the two-spotted goby, Gobiusculus flavescens

Table 2 Summary of parentage analysis data for Gobiusculus flavescens nests

Male ID	TL (mm)	WM (g)	Nest ID	Nest fullness^a	# larvae per nest	# larvae assayed	# females	# sneakers	# eggs sneaked
M01	48.0	0.79	C01	---	501	38	2	0	0
M02	40.5	0.48	C02	100	1005	42	3	0	0
M03	43.5	0.60	C03	90	3858	40	5	0	0
M04	44.5	0.64	C04	---	1789	81	4	0	0
M05	44.0	0.59	C05	80	2527	36	6	0	0
M06	41.5	0.50	C06	100	2241	41	4	0	0
M07	37.0	0.37	C07	100	3502	31	4	1	1
M08	46.0	0.74	C08^b	90	1660	---	---	---	---
M09	42.5	0.48	C09	100	5636	42	5	0	0
M10	40.0	0.52	C10	100	3183	37	5	0	0
M11	45.5	0.60	C11	50	1565	44	3	0	0
M12	40.0	0.43	C12	100	3217	36	5	0	0
M13	41.5	0.57	C13	100	3925	70	6	0	0
M14	38.0	0.37	C14	---	852	26	4	0	0
M15	43.0	0.59	C15	90	1197	37	4	0	0
M16	40.0	0.40	C16	100	1655	30	4	0	0
M17	46.5	0.69	C17	100	3882	41	5	0	0
M18	45.0	0.67	C18	---	2946	38	6	0	0
M19	45.0	0.68	C19	30	157	32	2	0	0
M20	41.5	0.53	C20	100	1784	42	5	0	0
M21	43.0	0.64	C21	---	754	46	3	0	0
M22	38.0	0.47	C22	90	2676	72	5	0	0

^aNest fullness was not estimated in non-mussel nests (C14, C21) or in nests hatching at time of collection (C01, C04, C18).
^bParentage analysis could not be completed on nest C08 due to poor PCR amplification.
Total length (TL) and wet body mass (WM) are shown for each male surveyed and the nest ID, percent fullness of the nest (nest fullness), number of offspring per nest (# larvae per nest), number of offspring genotyped (# larvae assayed), number of unique reconstructed female genotypes (# females), the number of sneaker males detected (# sneakers) and the number of assayed larva that were fertilized by sneakers (# sneaked).