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Table 2 Fluorescence and absorbance properties of amphioxus GFPs representative of five clades.

From: Amphioxus encodes the largest known family of green fluorescent proteins, which have diversified into distinct functional classes

GFP Abs. Max (nm) FWHM (nm) Ex. Max (nm) FWHM (nm) Em. Max (nm) FWHM (nm) Extinction Coefficient (M-1 cm-1) at 470 nm Extinction Coefficient (M-1 cm-1) at 500 nm Relative Fluorescence Intensity
GFPa1 497 45 500 38 516 39 48,100 120,900 100%
GFPe1 492 51 n.d. n.d. 524 56 56,000 130,700 0.31%
GFPf1 504 60 n.d. n.d. n.f. n.a. 13,400 25,500 n.a.
GFPd2 470 68 n.d. n.d. 495 57 60,600 6,100 0.35%
GFPc1 493 55 n.d. n.d. 521 56 63,900 98,800 0.33%
  1. Excitation maximum was determined for emission set at 516 nm. Emission maximum was determined upon excitation at 500 nm for GFPa1 and at 465 nm for GFPe1, GFPf1, GFPd2, and GFPc1. Relative fluorescence intensity values are shown relative to the most fluorescent GFP, GFPa1. Abbrev.:Abs., absorbance, Ex., Excitation, Em., Emission, FWHM; full width half max, n.f., non-fluorescent; n.a., not applicable.