Phylogenetic analysis, structural evolution and functional divergence of the 12-oxo-phytodienoate acid reductase gene family in plants

Table 3 Analysis of functional divergence between OPR subfamilies in plants

Comparison	θ₁ ± S.E^a	P ^b	Q_k>0.70^c	Critical amino acid sites^d
Sub. I vs II	0.220 ± 0.056	<0.01	7	35, 65, 70, 110, 258, 306, 324
Sub. I vs III	0.280 ± 0.055	<0.01	11	41, 54, 69, 80, 104, 243, 258, 312, 324, 330, 360
Sub. I vs IV	0.176 ± 0.070	<0.01	1	212
Sub. I vs V	0.351 ± 0.083	<0.01	7	62, 98, 166, 212, 227, 254*, 264
Sub. I vs VI	0.229 ± 0.063	<0.01	5	47, 110, 157, 212, 307
Sub. I vs VII	0.377 ± 0.077	<0.01	16	38, 43, 56, 62, 68, 85, 163, 178, 207, 227, 245, 257,258, 327*, 331, 332
Sub. II vs III	0.340 ± 0.064	<0.01	14	41, 65, 69, 70, 80, 85, 167, 169, 194, 201, 243, 306, 308, 309
Sub. II vs IV	0.001 ± 0.022	>0.05	0	Not found
Sub. II vs V	0.142 ± 0.133	>0.05	0	Not found
Sub. II vs VI	0.269 ± 0.064	<0.01	10	38, 65, 157, 191, 223, 245, 248, 307, 308, 324
Sub. II vs VII	0.114 ± 0.083	<0.05	0	Not found
Sub. III vs IV	0.350 ± 0.086	<0.01	8	21, 40, 83, 187, 254, 307, 308, 309
Sub. III vs V	0.437 ± 0.084	<0.01		16 62, 66, 70, 82, 104, 158, 176, 243, 254, 255, 258, 264, 273, 275, 318, 330*
Sub. III vs VI	0.353 ± 0.068	<0.01	13	21, 70, 87, 158, 243, 248, 255, 259, 309, 312, 314, 318, 324
Sub. III vs VII	0.378 ± 0.081	<0.01	12	56, 62, 68, 69, 85, 189, 205, 228, 233, 245, 324, 330*
Sub. IV vs V	0.288 ± 0.155	<0.05	0	Not found
Sub. IV vs VI	0.351 ± 0.083	<0.01	9	47, 157, 189, 223, 248, 254, 265, 307, 308
Sub. IV vs VII	0.259 ± 0.153	<0.05	0	Not found
Sub. V vs VI	0.159 ± 0.105	>0.05	0	Not found
Sub. V vs VII	0.316 ± 0.162	<0.05	2	264, 332
Sub. VI vs VII	0.178 ± 0.072	<0.01	1	245

^a θ is the coefficient of functional divergence; θ₁ ± S.E. is the coefficient of type I functional divergence between two clusters and its standard error.
^b The significance level (P value) is computed using Fisher's transformation.
^c Q_k, posterior probability. A site-specific profile based on the posterior probability (Q_k) was used to identify critical amino acid residues that were responsible for functional divergence.
^d Numbering of amino acid residues corresponds to AtOPR02-1. Critical amino acid sites with the highest posterior values (Q_k>0.70) are shown.
* The amino acid residues depicted with an asterisk were also found to be predicted in positive-selection sites between OPR paralogues (see Table 2).

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