Pigmentary and photonic coloration mechanisms reveal taxonomic relationships of the Cattlehearts (Lepidoptera: Papilionidae: Parides)
© Wilts et al.; licensee BioMed Central Ltd. 2014
Received: 2 April 2014
Accepted: 14 July 2014
Published: 27 July 2014
The colorful wing patterns of butterflies, a prime example of biodiversity, can change dramatically within closely related species. Wing pattern diversity is specifically present among papilionid butterflies. Whether a correlation between color and the evolution of these butterflies exists so far remained unsolved.
We here investigate the Cattlehearts, Parides, a small Neotropical genus of papilionid butterflies with 36 members, the wings of which are marked by distinctly colored patches. By applying various physical techniques, we investigate the coloration toolkit of the wing scales. The wing scales contain two different, wavelength-selective absorbing pigments, causing pigmentary colorations. Scale ridges with multilayered lamellae, lumen multilayers or gyroid photonic crystals in the scale lumen create structural colors that are variously combined with these pigmentary colors.
The pigmentary and structural traits strongly correlate with the taxonomical distribution of Parides species. The experimental findings add crucial insight into the evolution of butterfly wing scales and show the importance of morphological parameter mapping for butterfly phylogenetics.
The wing coloration and patterning of a butterfly play important roles in its everyday survival. The coloration pattern can function as a concealment or disguise signal for protection (camouflage, crypsis) or for attracting potential mates (display) ,. Coloration furthermore is an important aspect in mimicry, i.e. the parallel evolution of species that have evolved optically similar traits. Indeed, color is an important element of species selection mechanisms as many species have evolved by means of diverging coloration . Evolutionary mechanisms of coloration appeared independently in multiple phylogenetic places, contributing largely to the rich biodiversity of life present on earth today . Butterflies are a prime example of biodiversity ,. Even though extensively investigated in the past, it remains unclear whether their phylogeny and wing color are correlated.
Here, we investigate a possible color-phylogeny correlation in a small genus of papilionid butterflies, the Cattlehearts (Parides; Huebner, 1819), a Neotropical New World taxon with 36 members ,. The coloration of Parides butterflies is quite stunning and diverse (Figure 1). The wings are predominantly black, but distinct wing patches feature vividly colored scales that can strongly differ between species. The colors and arrangement of these brightly colored spots on the butterfly wings serve as an aposematic signal to birds . Aposematism is an antipredatory strategy where a potential prey radiates its unpalatability by a signal that is readily understood by predators . With their prominent color patterns, Parides butterflies are suitable for Müllerian mimicry rings. Their predators – especially birds – quickly learn to recognize these patterns and become averse to it .
Coloration is generally divided into two classes: pigmentary (chemical) coloration, due to wavelength-selective light absorption by pigments, and structural (physical) coloration, due to wavelength-selective light reflection resulting from incident light interacting with nanostructured matter . Up to date, of the Cattlehearts only the coloration principles of the Emerald-Patched Cattleheart, P. sesostris, have been elucidated in extensive detail –. The wing scales of the green patches at the dorsal wings of P. sesostris contain a single-network, gyroid-type photonic crystal layer, which reflects blue-green light, depending on the angle of illumination. However, the scales also contain a violet-absorbing pigment that functions as a spectral filter, causing an angle-independent, vivid-green wing color .
The small size of the genus with its large diversity in wing coloration makes the Parides an attractive study group for investigating possible color-phylogeny relationships. By applying various physical techniques , we investigated the coloration mechanisms of the male members of the genus Parides. We thus identified a restricted set of pigments and photonic structures that together create the vivid colors of Parides butterflies. We discuss the results in view of the evolution of butterfly wing scales and the taxonomic relationships of coloration in phylogenetic trees.
Appearance of the wings and wing scale morphology
The dorsal wings of P. quadratus (Figure 1A) are largely black with small yellow patches in the hindwings. As expected for its yellow color, the reflectance spectrum of the wing patches is low in the ultraviolet and high in the long-wavelength range (Figure 1B). The reflectance of the black wing areas is minimal in the ultraviolet and visible wavelength ranges but becomes noticeable in the far-red, which is characteristic for high concentrations of melanin (Figure 1B). The yellow scales are densely stacked in a regular lattice (Figure 1C). Scanning electron microscopy shows that the scales are built according to the basic bauplan of papilionid butterfly wing scales ,; the upper surface features parallel ridges, with in between randomly arranged cross-ribs, which form large windows (Figure 1D).
The dorsal forewings of P. aeneas bolivar (Figure 1I) have green patches and the hindwings red patches. Their reflectance spectra contrast strongly (Figure 1J). The quite pronounced green reflectance indicates a structural origin, which is also suggested by the gleam of the wing scales (Figure 1K). Indeed, scanning electron microscopy revealed elaborately structured scales (Figure 1L). The scale lumen consists of a large multilayer, and the scale’s upper lamina has tall ridges connected with a thick layer of cross-ribs, quite in contrast with the basic pattern of moderate ridges and thin cross-ribs (Figure 1D).
Of the species shown in Figure 1, P. childrenae oedippus (Figure 1M) has the most striking color pattern. The reflectance spectra of the green and red patches are similar to those of P. aeneas, suggesting similar coloration mechanisms. However, with epi-illumination light microscopy the green scales have a rather different optical appearance, especially a different gloss, indicating the presence of dissimilar photonic structures (Figure 1O). Scanning electron microscopy of a fractured scale revealed a 3D network-like structure in the scale lumen (Figure 1P), which was previously demonstrated to be a gyroid-type photonic crystal ,–.
The reflectance spectra of the yellow and red scale patches (Figure 1B,F,J,N) indicate the presence of two different, short-wavelength absorbing pigments. To identify the pigments, we immersed single scales in refractive index matching fluid (n = 1.55) and measured transmittance spectra with a microspectrophotometer (MSP) (Figure 3A). The differently colored scales yielded two classes of absorbance spectra (Figure 3A). The yellow, olive and green colored scales all contained a pigment with peak wavelength ~390 nm and all red scales contained a pigment with peak wavelength ~520 nm.
We also measured with the MSP reflectance spectra of small scale areas (diameter ~15 μm) of isolated single scales (Figure 3B). These spectra can be readily understood from the absorbance spectra of Figure 3A, because the pigments selectively absorb incident light in restricted wavelength ranges and thus the pigments reduce the amount of backscattered light. This is further illustrated by the scatterograms of a yellow and red scale. Illumination of a small scale area with a narrow-aperture white light beam results in both cases in a spatially wide angled, diffuse light distribution that virtually fills the whole hemisphere (Figure 3C,D).
Structural coloration mechanisms
Additionally to pigmentary coloration, three distinct forms of structural coloration occur in the wing scales of Parides butterflies: ridge multilayers (Figure 1H), lumen multilayers (Figure 1L) and gyroid-type photonic crystals (Figure 1P). We have investigated the consequences of these scale structures for light reflection with scatterometry and by measuring reflectance spectra as a function of illumination angle.
We further investigated the angle-dependence of the blue reflection using the ARM setup, consisting of two optical fibers that can be rotated around the sample (see Methods). We first aligned the illumination and detection fiber and determined the angle where the reflectance was maximal. This was called the normal position. We then measured reflectance spectra as a function of the angle of light incidence relative to the normal position, Δα, whilst the detection angle, Δβ, was set at the mirror position: Δβ = −Δα (Figure 2E). When increasing the angle of incidence, the reflectance shifted hypsochromically, i.e. towards shorter wavelengths, as expected for a multilayer ,. We compared the angle-dependent shift in peak wavelength derived from Figure 2E, plotted in the inset, with values following from the interference condition for reflecting multilayers (see Methods) by using data for the thickness of the air (da) and cuticle layers (dc) estimated by scanning electron microscopy: da ≈ 82 nm and dc ≈ 105 nm (blue line). With refractive indices na = 1 and nc = 1.56 a reasonable correspondence was obtained (Figure 2E, inset). The reflectance amplitude decreased with increasing angle, which is presumably due to the small size of the multilayers.
The scatterogram of the upperside shows a rather wide-field green scattering plus a clear indication of diffraction by the parallel array of ridges (Figure 4A). The scatterogram of the scale underside shows blue scattered light in a restricted spatial field (Figure 4B). The latter must represent the direct multilayer reflection. The slight spatial spread can be immediately understood from SEM observations that the scale underside is only approximately (but not perfectly) flat.
Reflectance measurements with the MSP yielded a narrow-band, green-peaking spectrum for the upperside and a much broader and more symmetrical blue-green-peaking spectrum for the underside (Figure 4C). Considering the scale’s anatomy (Figure 1L), the obvious interpretation of the difference in the reflectance spectra is that the spectrum of the underside is virtually fully determined by the multilayer reflector in the scale lumen, whilst the spectrum measured from the upperside represents the multilayer’s reflectance spectrum filtered by the violet-absorbing pigment.
The multilayer reflectance properties can be modeled when the layer thicknesses, the refractive indices and the number of layers are known. From SEM images we estimated the layer thickness as ~80 nm and the number of cuticle layers to be 7. The individual layers appeared to be not entirely uniform. The chitin layers are separated by air layers, but they are connected by tiny pillars, the trabeculae, raising the effective refractive index of the air layers to >1. Furthermore, the chitin layers are not smooth, but locally punctured with air holes, which reduces the effective refractive index of the cuticle layers to <1.56. Using refractive index values na = 1.09 and nc = 1.45 (i.e. a cuticle filling fraction of 0.8), we could well reproduce the measured dependence of the reflectance peak wavelength on the angle of light incidence (Figure 5C and Additional file 1: Figure S2).
Gyroid-type photonic crystals
The striking green wing patches of e.g. P. childrenae and P. sesostris have scales with a 3D photonic crystal in the scale lumen with in the upper lamina a honeycomb-structured layer containing the violet-absorbing pigment (Figure 1L; see also ). The photonic crystal layer consists of a number of differently-oriented gyroid interference reflectors, reflecting predominantly in the blue-green wavelength range –. The broader reflectance spectrum of the gyroid reflector is narrowed to the observed vivid green due to the violet-absorbing pigment in the honeycomb layer , in a way that is surprisingly similar to that described above for the pigmented scales with a multilayer in the lumen.
The different coloration mechanisms present in the Cattlehearts can be related to their phylogeny. The taxonomy of the family Papilionidae is generally well investigated, but only recently the genus Parides has been categorized by molecular techniques on the species-level with a limited number of species (18 out of 36 species). Phylogenetic information on the species-level for Parides butterflies has been published by Silva-Brandao et al.  based on DNA barcoding, and later slightly adjusted by Condamine et al. . Further, Möhn et al.  classified the Cattlehearts into four major lineages depending on DNA results, habitat, external characteristics and color appearance.
For the structural parameters, however, the mapping shows significant differences. The classification by Condamine et al. , the most finely sampled phylogeny to date (Figure 6A), divides the Cattlehearts in four groups. The butterflies in groups 1 and 2 do not have a special photonic structure, but those of group 3 have either a ridge multilayer or a gyroid photonic crystal. Most of the group 4 butterflies have a multilayer in the scale lumen. The phylogeny suggests multiple independent occurrences and losses of structural characters. This is specifically obvious in the occurrence (and loss) of the ridge multilayer structures and the gyroid-type photonic crystals, which in the current phylogeny appear to have evolved and got lost multiple times within group 3. This is a highly unlikely scenario, especially when considering the (potential) close relationship in the wing scale development of perforated multilayer structures and gyroids photonic crystals in the scale lumen (see below).
The classification by Möhn et al.  is rather coarsely sampled (Figure 6B). The structural parameters mostly occur in one specific group only, except for the ascanius species-group where both ridge and lumen multilayers occur. This supports a single evolutionary occurrence of these structures, much in contrast to the classification of Figure 6A. Especially in comparison with the finer sampled tree of Figure 6A, it seems likely that there has been a unique development of the trait rather than multiple independent occurrences. Thus, a species-level tree is necessary to draw specific conclusions on phylogeny.
Figure 6C presents an alternative phylogeny, based on the pigments and the morphological characters we identified. Albeit not being based on maximum parsimony or bootstrap methods, the tree minimizes the individual evolution of identical parameters. It will be interesting to investigate which result a combined phylogenetic analysis of morphological parameters and DNA barcoding would deliver .
Coloration mechanisms in the Cattlehearts
Various coloration mechanisms are realized in the wing scales of Parides butterflies. Four different cases, each with a unique nanostructure, can be identified: i) the basic bauplan of papilionid butterflies, with a flat lower lamina and a structured upper lamina with ridges and irregular cross-ribs ; ii) tilted ridge lamellar stacks acting as multilayer reflectors, similar to those observed in Morpho and Pieris butterflies ,,; iii) perforated multilayer stacks in the scale lumen similar to those in lycaenid butterflies ,, and iv) gyroid photonic crystal layers – as also observed in lycaenids ,,.
Pigments in the scales affect the scales’ reflectance spectra. The ubiquitous pigment melanin, which has an absorption spectrum extending well beyond the visible wavelength range, causes brown or black scales, depending on the pigment concentration . We identified two wavelength-selective absorbing pigments embedded in the cover scales of the different Cattleheart butterflies (Figure 3). The yellow scales of P. quadratus and the green scales of several Cattlehearts contain an (ultra)violet-absorbing pigment, which is most likely the well-known papiliochrome II of other papilionids ,,. The red scales of several Cattlehearts contain a blue-green-absorbing pigment, which has not been specifically characterized but presumably is also a papiliochrome type pigment . The pigments are located (at least partly) in the upper lamina (Additional file 1: Figure S1), which is profuse in those cases where the scale lumen contains a photonic structure, a multilayer or a 3D gyroid-type photonic crystal. Both photonic structures specifically reflect blue-green light, and in both cases the reflectance spectra are narrowed down to the green wavelength range due to spectral filtering by the pigment . The upper lamina is irregular structured and thus removes the intrinsic directionality of the reflected light and makes the color mostly angle-independent (Figure 5).
We found that the scales in some Cattlehearts are distinctly curved (Figure 2). This suggests that scale curvature also tunes the directionality of the reflected light (see also ). Furthermore, the jet-black framing, due to heavily melanized scales, creates a contrastful wing pattern, strongly increasing the visibility of the colored wing patches, for instance for potential predators or mates.
Wing scale development
Closely related Parides species can have scales with in the lumen either perforated multilayer structures (P. aeanas and others) or gyroid photonic crystals (P. childrenae and P. sesostris, see Additional file 2: Table S1 and Figures 4, and 6). From a developmental point-of-view, it is rather difficult to assess and visualize the different stages of wing scale development. However, the self-assembly of block-copolymers can be seen as an analogue model for the development of wing scales ,. In block-copolymer systems perforated lamellar and gyroid structured phases are stable configurations lying close to each other in the phase diagram ,. It is hence very likely that in the developing cell that develops into the butterfly wing scale, the perforated multilayers and gyroid photonic crystals are formed due to slightly different development conditions. A change in the developmental conditions, similar to e.g. a change in the concentration resulting in a phase transition in polymers , may thus be enough to ‘switch’ between the development of a perforated multilayer or a gyroid in the scale lumen.
This is the first observation of such a close connection between the lamellar phase (perforated multilayers) and the gyroid phase (photonic crystals) between two closely related species in the same genus. Additional research into the wing scale development of the Cattlehearts might therefore give important physical and chemical insight into how these complex optical structures are formed, for instance by cell-mediated self-assembly.
Wing pattern and mimicry
Many male Cattlehearts feature a similar wing patterning, and curiously, the colors of wing patches due to a multilayer or a gyroid photonic crystal in the scale lumen are nearly indistinguishable. This suggests a function of these wing patterns in mimicry rings and/or aposematic signaling, especially since many (strongly colored) Cattlehearts, like P. sesostris, are shunned by birds, their main predators .
We conclude that our experimental observations on the pigmentation and photonic structures in the wing scales of the Cattlehearts add important insight into the evolution of butterfly wing scales, especially given the simultaneous observation of perforated multilayers and gyroids in wing scales of butterflies within the same genus. This shows that in addition to advanced phylogenetic tools based on DNA barcoding , the pigmentation and morphological parameters of butterfly wing scales provide an important instrument for a detailed analysis of phylogenetic trees. Especially the highly unlikely appearance of multiple traits in closely related butterflies (Figure 6A) should be taken into account when assembling maximum likelihood phylogenetic trees and incurring on the evolution of butterflies and specifically butterfly wing traits ,.
Specimens of the butterflies were obtained commercially trough Tropical Butterflies and Insects of America (Tampa, FL, USA) and The Bug Maniac (Makassar, Indonesia). We furthermore investigated Parides butterflies in the Lepidoptera collection of the Natural History Museum Naturalis (Leiden, the Netherlands). Additional file 2: Table S1 summarizes the investigated specimens.
The butterflies were photographed with a Canon EOS 550D camera (Canon Inc., Tokyo, Japan) equipped with a Canon 50 mm-macro objective and a ring flash. Details of the scale arrangement on the wings were photographed with a Zeiss Universalmikroskop (Carl Zeiss AG, Oberkochen, Germany) equipped with a Kappa DX-40 digital camera (Kappa GmbH, Gleichen, Germany).
Reflectance spectra of intact wings were measured with a bifurcated probe (Avantes FCR-7UV200, Avantes, Eerbeek, The Netherlands). The probe delivered light about normally to the wing plane and the also about normally reflected light was channeled to a photodiode array spectrometer (AvaSpec-2048-2, Avantes). The light source was a Deuterium-Halogen lamp (Avantes D(H)-S). A white diffuse reference tile (Avantes WS-2) served as a reference in all reflectance measurements. Transmittance spectra obtained from isolated wing scales, immersed in a fluid with refractive index 1.55 (Cargille Labs, Cedar Grove, NJ, USA), were measured with a microspectrophotometer (MSP), being a Leitz-Ortholux microscope, equipped with the Avantes light source and spectrometer. The measured transmittance, T(λ), yielded the absorbance (or optical density) D(λ) = − log10T(λ), where λ is the wavelength.
The angular distribution of light scattered by intact wings was measured with an angle-dependent reflectance measurement (ARM) setup, which consists of two optical fibers, mounted at two independent rotatable, but coaxial goniometers. A focused Xenon light source delivered light via one fiber to an area with diameter ~4 mm. The other fiber collected the reflected and scattered light from a wing area with diameter ~9 mm and delivered it to the Avantes spectrometer. A UV/VIS-polarizer was mounted in front of the detection fiber (see also ,). The wings were placed on a black cardboard and positioned at the collinear axes of rotation of the two goniometers. The white diffuse reference tile served as a reference.
Imaging scatterometry (ISM)
The (far-field) spatial scattering pattern of single wing scales, glued to the end of a pulled glass micropipette, was investigated with an imaging scatterometer (ISM; ,,). The ISM is built around an ellipsoidal mirror, collecting light from a full hemisphere. The investigated objects, i.e. the wing scales, positioned in the mirror’s first focal point, were illuminated either with a narrow-aperture primary beam via a small hole in the ellipsoidal mirror or with a large-aperture secondary beam via a half-mirror. The far-field scattering pattern of the light backscattered by the sample was collected by a digital camera. Angle-dependent reflectance spectra were collected via a half-mirror using the Avantes spectrometer ,. A small piece of magnesium oxide served as a white diffuse reference object.
Scanning electron microscopy (SEM)
The structure of the wing scales was investigated with a Philips XL30-ESEM scanning electron microscope (SEM). To prevent electric charging, the samples were sputtered with gold or palladium prior to imaging.
The reflectance of the wing scales was modeled by applying multilayer theory . The ridges responsible for the scattering of light consist of alternating air (a) and cuticle (c) layers, with thickness da and dc and refractive index na and nc. Although the cuticle refractive index is slightly dispersive , we have assumed for all wavelengths na =1.0 and n c = 1.56, to simplify the analysis. The propagation of a light beam through the multilayer can be found from Snell’s law: na sin θa = nc sin θc, where θa and θc are the angles of incidence in air and cuticular medium. The wavelength of peak reflectance of a multilayer is given by the interference condition λmax = 2(nada cos θ a + ncdc cos θc) ,.
We have used the species-level taxonomy provided by Condamine et al. (; slightly updated from ), where 17 species have been examined on the species-level by DNA barcoding, and the taxonomy provided by Möhn et al. , where 36 species have been grouped (for more details, see Additional file 1).
The data set supporting the results of this article is included within the article and its additional files.
We thank Zsolt Bálint, Casper van der Kooi and two anonymous reviewers for valuable comments on the manuscript and Hein Leertouwer for collaboration. This study was financially supported by the Air Force Office of Scientific Research/European Office of Aerospace Research and Development (Grant FA8655-08-1-3012 to D.G.S.).
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