Environment-dependent microevolution in a Mediterranean pine (Pinus pinasterAiton)

Table 2 Mixed model analysis of two experiments (under controlled and semi-natural conditions) in maritime pine, including fixed effects (F-value and significance level) and covariates (DW: dry weight, WSEED: seed weight)

Trait	Environment-controlled conditions(Experiment 1)						Outdoor semi-natural conditions(Experiment 2)
	F-value, significance level¹			%Var	Mean²		F-value, significance level¹			%Var	Mean²
	TRT	BLOCK	COV	TRT*FAM	W1	W2	TRT	BLOCK	COV	TRT*FAM	D1	D2	Covariate
LMF	28.70 ***	7.43 **	20.19 ***	0.0 ns	0.431	0.486	0.04 ns	12.14 ***	58.01 ***	1.4 ns	0.477	0.461	DW
SMF	9.00 **	1.81 ns	0.61 ns	3.7 ns	0.054	0.059	0.01 ns	8.71 **	24.73 ***	0.0 ns	0.161	0.165	DW
RMF	35.24 ***	7.96 **	16.16 ***	0.0 ns	0.514	0.454	0.03 ns	10.85 ***	19.60 ***	1.0 ns	0.361	0.379	DW
DW	280.23 ***	62.17 ***	29.62 ***	0.0 ns	0.7406	1.0519	7.13 **	4.69 **	9.08 **	0.0 ns	0.4257	0.5725	WSEED
SLA	77.76 ***	26.80 ***	1.21 ns	0.0 ns	49.59	56.70	12.26 ***	3.67 *	1.72 ns	2.8 ns	84.99	79.16	WSEED
NSU	40.56 ***	40.90 ***	15.82 **	3.8 ns	36.53	45.30	2.27 ns	1.99 ns	9.71 **	0.0 ns	82.13	90.88	WSEED
HON3	7.73 **	0.28 ns	3.16 ns	2.6 ns	25.91	27.61	21.80 ***	6.60 **	9.10 **	0.0 ns	26.07	29.45	WSEED
b	0.03 ns	1.06 ns	5.22 *	0.0 ns	0.0637	0.0663	NA	NA	NA	NA	NA	NA	WSSED
m	101.79 ***	3.57 *	10.59 **	2.6 ns	191.3	204.2	NA	NA	NA	NA	NA	NA	WSEED
HTOT	159.48 ***	4.48 *	5.01 *	0.0 ns	34.47	42.45	8.73 **	2.55 ns	16.60 ***	0.0 ns	44.82	50.31	WSEED
SUR	14.49 **	7.11 **		4.4 ns	0.827	0.594	0.08 ns	1.22 ns		0.0 ns	0.722	0.697

TRT: treatment (W1/W2, D1/D2; see Methods); COV: covariate;%Var: Percent of the interaction term (TRT*FAM) with respect to the total variance; LMF: leaf mass fraction; SMF: stem mass fraction; RMF: root mass fraction; DW: dry weight; SLA: specific leaf area; NSU: number of stem units; HON3: height at first production of axillary shoots; b, m: coefficients of the Gompertz function; HTOT: total height; SUR: survival; NA: not available.
¹ns: not significant, *: 0.05 ≤ α<?0.01, **: 0.01 ≤ α< 0.001, ***: α ≤ 0.001.
²Best linear unbiased estimates (BLUE) for watering (W1 and W2) and density (D1 and D2).

ISSN: 2730-7182