Results of biogeographic analysis. Historical biogeography reconstruction using the Statistical Dispersal-Vicariance Analysis (S-DiVA) A) Topology of the bayesian phylogenetic tree with mapped distribution area and dispersal & vicariance events. Age of nodes with vicariant event corresponds to the timeline (after BEAST analysis). A-J represents the biogeographic regions used for S-DiVA analysis based following with slight modifications) the proposed African freshwater ecoregion (Abell et al., ). See the unit description in the legend on the right side of the figure, as well as on the schematic Africa map. B) The schematic graphics of the four Nothobranchius clades and their allopatric distribution. C) The proposed colonization events older than 4 Mya (bold line) and younger than 4 Mya (dashed line).