Figure 4
From: Neofunctionalization of a duplicate hatching enzyme gene during the evolution of teleost fishes
SDS-PAGE of unfertilized egg envelopes and their digests following treatment with MfHEs. Egg envelopes isolated from unfertilized eggs of the milkfish (lane 1) were digested by rMfHE1 for 15, 30 or 60 min (lane 2–4), or rMfHE3 for 30 or 60 min (lane 5, 6). The numbers on the right represent the sizes of the major bands. The numbers on the left represent the sizes of the molecular markers.