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Figure 5 | BMC Evolutionary Biology

Figure 5

From: Neofunctionalization of a duplicate hatching enzyme gene during the evolution of teleost fishes

Figure 5

The cleavage sites of MfHE1 and MfHE3, and schematic diagram of predicted products. (A) amino acid sequences predicted from cDNA of milkfish ZPB and ZPCa, and the cleavage sites of MfHEs. The black and grey arrowheads indicate the cleavage sites of MfHE1 and MfHE3, respectively. The ZP domains and trefoil domain are shown in light and dark gray boxes, respectively. The black triangles are putative cleavage sites of the signal peptidase. The white triangle represents C-terminal processing sites. (B) Schematic diagrams of MfZPB and MfZPCa, and the cleavage sites of MfHE1 and MfHE3. The predicted molecular sizes of the products produced by MfHE1 and MfHE3 are shown as black boxes. ZP-N and ZP-C indicate the two subdomains in the ZP domain. The small circle in MfZPB indicates the trefoil domain. (C) Alignment of the amino acid sequences of ZPC around the N-ZPd site of milkfish (MfZPCa), zebrafish (zfZP3), killifish (FhChgL), and medaka (OlChgL). Identical residues are boxed and dashes represent gaps. The gray arrowheads indicate the cleavage sites for each fish hatching enzyme in this region. The black arrow heads indicate the N-ZPd sites specifically cleaved by clade II enzymes, MfHE3, FLCE, and MLCE. The amino acid sequence is numbered from the N-terminus of the ZP domain.

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